验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HeLa cells, human heart tissue, Jurkat cells, K-562 cells, mouse heart tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13015-1-AP targets HIP55 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IP, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | HIP55 fusion protein Ag4078 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | drebrin-like |
| 别名 | DBNL, HPK1-interacting protein of 55 kDa, Drebrin-like protein, Drebrin-F, Cervical mucin-associated protein |
| 计算分子量 | 430 aa, 48 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC031687 |
| 基因名称 | HIP55 |
| Gene ID (NCBI) | 28988 |
| RRID | AB_2230306 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UJU6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HIP55, also known as DBNL, ABP1, SH3P7, belongs to the ABP1 family. HIP55 plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. HIP55 acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes (PMID: 14729663). The N terminus of HIP55 contains a putative actin-binding domain found in drebrins, which are involved in brain development, and the C terminus contains an SH3 domain. Expect a band 55 kDa in size corresponding to HIP55 by western blotting.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HIP55 antibody 13015-1-AP | Download protocol |
| IHC protocol for HIP55 antibody 13015-1-AP | Download protocol |
| IF protocol for HIP55 antibody 13015-1-AP | Download protocol |
| IP protocol for HIP55 antibody 13015-1-AP | Download protocol |
| FC protocol for HIP55 antibody 13015-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Acta Pharmacol Sin The multifunctional adaptor protein HIP-55 couples Smad7 to accelerate TGF-β type I receptor degradation.
| ||
J Neurosci Drebrin-like (Dbnl) controls neuronal migration via regulating N-cadherin expression in the developing cerebral cortex. | ||
J Cell Sci VEGF-A stimulates podosome-mediated collagen-IV proteolysis in microvascular endothelial cells. | ||
J Microbiol Effect of Salmonella treatment on an implanted tumor (CT26) in a mouse model. | ||
