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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated HIP55 Polyclonal antibody

HIP55 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-13015

Print datasheet

Synonyms

ABP1, Cervical SH3P7, CMAP, DBNL, Drebrin F, drebrin like, Drebrin like protein, HIP 55, SH3P7

验证数据展示

  • IF Staining of HepG2 using CL488-13015

    IF Staining of HepG2 using CL488-13015

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 HIP55 antibody (CL488-13015) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 13015-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13015 targets HIP55 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen HIP55 fusion protein Ag4078 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name drebrin-like
Synonyms ABP1, Cervical SH3P7, CMAP, DBNL, Drebrin F, drebrin like, Drebrin like protein, HIP 55, SH3P7
Calculated Molecular Weight 430 aa, 48 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC031687
Gene Symbol HIP55
Gene ID (NCBI) 28988
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJU6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIP55, also known as DBNL, ABP1, SH3P7, belongs to the ABP1 family. HIP55 plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. HIP55 acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes (PMID: 14729663). The N terminus of HIP55 contains a putative actin-binding domain found in drebrins, which are involved in brain development, and the C terminus contains an SH3 domain. Expect a band 55 kDa in size corresponding to HIP55 by western blotting.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HIP55 antibody CL488-13015Download protocol
Standard Protocols
Click here to view our Standard Protocols