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验证数据展示

  • WB analysis of human brain using 16019-1-AP (same clone as 16019-1-PBS)

    WB analysis of human brain using 16019-1-AP (same clone as 16019-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 16019-1-AP (DBR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 16019-1-AP (same clone as 16019-1-PBS)

    WB analysis of HeLa using 16019-1-AP (same clone as 16019-1-PBS)

    WB result of DBR1 antibody (16019-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DBR1 transfected HeLa cells. This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 16019-1-AP (same clone as 16019-1-PBS)

    IP experiment of mouse brain using 16019-1-AP (same clone as 16019-1-PBS)

    IP result of anti-DBR1 (IP:16019-1-AP, 4ug; Detection:16019-1-AP 1:500) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 16019-1-AP (same clone as 16019-1-PBS)

    IHC staining of human placenta using 16019-1-AP (same clone as 16019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 16019-1-AP (DBR1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 16019-1-AP (same clone as 16019-1-PBS)

    IHC staining of human placenta using 16019-1-AP (same clone as 16019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 16019-1-AP (DBR1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 16019-1-AP (same clone as 16019-1-PBS)

    IF Staining of HeLa using 16019-1-AP (same clone as 16019-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DBR1 antibody (16019-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

DBR1 Polyclonal antibody, PBS Only

DBR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16019-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

16019-1-PBS targets DBR1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 DBR1 fusion protein Ag8830 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 debranching enzyme homolog 1 (S. cerevisiae)
别名
计算分子量 544 aa, 62 kDa
观测分子量 70-80 kDa
GenBank蛋白编号BC009472
基因名称 DBR1
Gene ID (NCBI) 51163
RRIDAB_2230316
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UK59
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DBR1(Lariat debranching enzyme) hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules. DBR1 belongs to the lariat debranching enzyme family. Inhibiton of Dbr1 can suppress TDP-43 toxicity in primary neurons, suggesting that Dbr1 could be a potential therapeutic target for ALS and related TDP-43 proteinopathies (PMID: 23104007). This protein has 2 isoforms produced by alternative splicing.