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验证数据展示

  • WB analysis of HeLa using 66951-1-Ig (same clone as 66951-1-PBS)

    WB analysis of HeLa using 66951-1-Ig (same clone as 66951-1-PBS)

    WB result of DBR1 antibody (66951-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DBR1 transfected HeLa cells. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • WB analysis using 66951-1-Ig (same clone as 66951-1-PBS)

    WB analysis using 66951-1-Ig (same clone as 66951-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 66951-1-Ig (same clone as 66951-1-PBS)

    IP experiment of HeLa using 66951-1-Ig (same clone as 66951-1-PBS)

    IP result of anti-DBR1 (IP:66951-1-Ig, 4ug; Detection:66951-1-Ig 1:2000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66951-1-Ig (same clone as 66951-1-PBS)

    IHC staining of mouse brain using 66951-1-Ig (same clone as 66951-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66951-1-Ig (DBR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66951-1-Ig (same clone as 66951-1-PBS)

    IHC staining of mouse brain using 66951-1-Ig (same clone as 66951-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66951-1-Ig (DBR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 66951-1-Ig (same clone as 66951-1-PBS)

    IF Staining of A431 using 66951-1-Ig (same clone as 66951-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DBR1 antibody (66951-1-Ig, Clone: 3B5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

DBR1 Monoclonal antibody, PBS Only

DBR1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66951-1-PBS

产品说明书

CloneNo. 3B5A7

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat, Pig

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

DBR1, Lariat debranching enzyme

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66951-1-PBS targets DBR1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原 DBR1 fusion protein Ag8830 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 debranching enzyme homolog 1 (S. cerevisiae)
别名 DBR1, Lariat debranching enzyme
计算分子量 544 aa, 62 kDa
观测分子量 70-80 kDa
GenBank蛋白编号BC009472
基因名称 DBR1
Gene ID (NCBI) 51163
RRIDAB_2882274
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UK59
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DBR1(Lariat debranching enzyme) hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules. DBR1 belongs to the lariat debranching enzyme family. Inhibiton of Dbr1 can suppress TDP-43 toxicity in primary neurons, suggesting that Dbr1 could be a potential therapeutic target for ALS and related TDP-43 proteinopathies (PMID: 23104007). This protein has 2 isoforms produced by alternative splicing.