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验证数据展示

  • WB analysis of HeLa using 66951-1-Ig (same clone as 66951-1-PBS)

    WB analysis of HeLa using 66951-1-Ig (same clone as 66951-1-PBS)

    WB result of DBR1 antibody (66951-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DBR1 transfected HeLa cells. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • WB analysis using 66951-1-Ig (same clone as 66951-1-PBS)

    WB analysis using 66951-1-Ig (same clone as 66951-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 66951-1-Ig (same clone as 66951-1-PBS)

    IP experiment of HeLa using 66951-1-Ig (same clone as 66951-1-PBS)

    IP result of anti-DBR1 (IP:66951-1-Ig, 4ug; Detection:66951-1-Ig 1:2000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66951-1-Ig (same clone as 66951-1-PBS)

    IHC staining of mouse brain using 66951-1-Ig (same clone as 66951-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66951-1-Ig (DBR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66951-1-Ig (same clone as 66951-1-PBS)

    IHC staining of mouse brain using 66951-1-Ig (same clone as 66951-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66951-1-Ig (DBR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 66951-1-Ig (same clone as 66951-1-PBS)

    IF Staining of A431 using 66951-1-Ig (same clone as 66951-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DBR1 antibody (66951-1-Ig, Clone: 3B5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DBR1 Monoclonal antibody, PBS Only

DBR1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66951-1-PBS

Print datasheet

CloneNo. 3B5A7

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

DBR1, Lariat debranching enzyme

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

66951-1-PBS targets DBR1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen DBR1 fusion protein Ag8830 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name debranching enzyme homolog 1 (S. cerevisiae)
Synonyms DBR1, Lariat debranching enzyme
Calculated Molecular Weight 544 aa, 62 kDa
Observed Molecular Weight 70-80 kDa
GenBank Accession NumberBC009472
Gene Symbol DBR1
Gene ID (NCBI) 51163
RRIDAB_2882274
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UK59
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DBR1(Lariat debranching enzyme) hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules. DBR1 belongs to the lariat debranching enzyme family. Inhibiton of Dbr1 can suppress TDP-43 toxicity in primary neurons, suggesting that Dbr1 could be a potential therapeutic target for ALS and related TDP-43 proteinopathies (PMID: 23104007). This protein has 2 isoforms produced by alternative splicing.