验证数据展示
经过测试的应用
Positive WB detected in | human brain tissue, HeLa cells, MCF-7 cells, fetal human brain tissue, pig brain tissue, rat brain tissue, mouse brain tissue, HepG2 cells |
Positive IP detected in | HeLa cells, mouse brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A431 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66951-1-Ig targets DBR1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Tested Reactivity | Human, Mouse, Rat, Pig |
Immunogen | DBR1 fusion protein Ag8830 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | debranching enzyme homolog 1 (S. cerevisiae) |
Synonyms | DBR1, Lariat debranching enzyme |
Calculated Molecular Weight | 544 aa, 62 kDa |
Observed Molecular Weight | 70-80 kDa |
GenBank Accession Number | BC009472 |
Gene Symbol | DBR1 |
Gene ID (NCBI) | 51163 |
RRID | AB_2882274 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9UK59 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DBR1(Lariat debranching enzyme) hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules. DBR1 belongs to the lariat debranching enzyme family. Inhibiton of Dbr1 can suppress TDP-43 toxicity in primary neurons, suggesting that Dbr1 could be a potential therapeutic target for ALS and related TDP-43 proteinopathies (PMID: 23104007). This protein has 2 isoforms produced by alternative splicing.
实验方案
Product Specific Protocols | |
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WB protocol for DBR1 antibody 66951-1-Ig | Download protocol |
IHC protocol for DBR1 antibody 66951-1-Ig | Download protocol |
IF protocol for DBR1 antibody 66951-1-Ig | Download protocol |
IP protocol for DBR1 antibody 66951-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |