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验证数据展示

  • WB analysis of HeLa using 66951-1-Ig

    WB analysis of HeLa using 66951-1-Ig

    WB result of DBR1 antibody (66951-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DBR1 transfected HeLa cells.

  • WB analysis using 66951-1-Ig

    WB analysis using 66951-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 66951-1-Ig

    IP experiment of HeLa using 66951-1-Ig

    IP result of anti-DBR1 (IP:66951-1-Ig, 4ug; Detection:66951-1-Ig 1:2000) with HeLa cells lysate 1560 ug.

  • IHC staining of mouse brain using 66951-1-Ig

    IHC staining of mouse brain using 66951-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66951-1-Ig (DBR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 66951-1-Ig

    IHC staining of mouse brain using 66951-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66951-1-Ig (DBR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 66951-1-Ig

    IF Staining of A431 using 66951-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DBR1 antibody (66951-1-Ig, Clone: 3B5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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DBR1 Monoclonal antibody

DBR1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 66951-1-Ig

产品说明书

CloneNo. 3B5A7

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat, Pig

应用

WB, IHC, IF/ICC, IP, ELISA

别名

DBR1, Lariat debranching enzyme

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, HeLa cells, MCF-7 cells, fetal human brain tissue, pig brain tissue, rat brain tissue, mouse brain tissue, HepG2 cells
Positive IP detected inHeLa cells, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66951-1-Ig targets DBR1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原 DBR1 fusion protein Ag8830 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 debranching enzyme homolog 1 (S. cerevisiae)
别名 DBR1, Lariat debranching enzyme
计算分子量 544 aa, 62 kDa
观测分子量 70-80 kDa
GenBank蛋白编号BC009472
基因名称 DBR1
Gene ID (NCBI) 51163
RRIDAB_2882274
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UK59
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DBR1(Lariat debranching enzyme) hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules. DBR1 belongs to the lariat debranching enzyme family. Inhibiton of Dbr1 can suppress TDP-43 toxicity in primary neurons, suggesting that Dbr1 could be a potential therapeutic target for ALS and related TDP-43 proteinopathies (PMID: 23104007). This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for DBR1 antibody 66951-1-IgDownload protocol
IHC protocol for DBR1 antibody 66951-1-IgDownload protocol
IF protocol for DBR1 antibody 66951-1-IgDownload protocol
IP protocol for DBR1 antibody 66951-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols