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CoraLite® Plus 488-conjugated DBR1 Monoclonal antibody
DBR1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Mouse, Rat, Pig
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3B5A7
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A431 cells |
For other applications, we recommend the unconjugated version of this antibody, 66951-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66951 targets DBR1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Immunogen | DBR1 fusion protein Ag8830 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | debranching enzyme homolog 1 (S. cerevisiae) |
| Synonyms | DBR1, Lariat debranching enzyme |
| Calculated Molecular Weight | 544 aa, 62 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | BC009472 |
| Gene Symbol | DBR1 |
| Gene ID (NCBI) | 51163 |
| RRID | AB_2919395 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UK59 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DBR1(Lariat debranching enzyme) hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules. DBR1 belongs to the lariat debranching enzyme family. Inhibiton of Dbr1 can suppress TDP-43 toxicity in primary neurons, suggesting that Dbr1 could be a potential therapeutic target for ALS and related TDP-43 proteinopathies (PMID: 23104007). This protein has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 DBR1 antibody CL488-66951 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |