CoraLite® Plus 488-conjugated DCLK1 Monoclonal antibody
DCLK1 Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat, rabbit, pig
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3C12C1
验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
For other applications, we recommend the unconjugated version of this antibody, 68234-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68234 targets DCLK1 in IF-P applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | Human, mouse, rat, rabbit, pig |
| Immunogen | DCLK1 fusion protein Ag17110 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | doublecortin-like kinase 1 |
| Synonyms | DCAMKL1, DCDC3A, DCLK, DCLK1, doublecortin like kinase 1, KIAA0369 |
| Calculated Molecular Weight | 729 aa, 81 kDa |
| Observed Molecular Weight | 46 kDa, 82 kDa |
| GenBank Accession Number | BC152456 |
| Gene Symbol | DCLK1 |
| Gene ID (NCBI) | 9201 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O15075 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DCLK1 (Serine/threonine-protein kinase DCLK1) is also named as DCAMKL1, DCDC3A, KIAA0369 and belongs to the CAMK Ser/Thr protein kinase family. It is a microtubule-associated kinase that can undergo autophosphorylation and it also has microtubule-polymerizing activity that is independent of its protein kinase activity (PMID: 11124993). It plays a unique role in mitotic spindle integrity during early neurogenesis in radial glial cell proliferation and their radial process stability. DCLK1 is a unique marker for distinguishing tumor stem cells from intestinal normal stem cells (PMID: 23202126). This protein has 4 isoforms produced by alternative splicing with the molecular weight of 82 kDa, 81 kDa, 47 kDa and 48 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 DCLK1 antibody CL488-68234 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |