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Decorin Recombinant antibody, PBS Only

Decorin Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240781E3

Cat no : 83753-4-PBS

Print datasheet

Synonyms

DCN, PG40, PG S2, DSPG2, CSCD

验证数据展示

  • WB analysis using 83753-4-RR (same clone as 83753-4-PBS)

    WB analysis using 83753-4-RR (same clone as 83753-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83753-4-RR (Decorin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83753-4-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83753-4-RR (same clone as 83753-4-PBS)

    FC experiment of HepG2 using 83753-4-RR (same clone as 83753-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Decorin Recombinant antibody (83753-4-RR, Clone:240781E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83753-4-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 83753-4-RR (same clone as 83753-4-PBS)

    FC experiment of HEK-293 using 83753-4-RR (same clone as 83753-4-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Decorin Recombinant antibody (83753-4-RR, Clone:240781E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83753-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83753-4-RR

    Affinity and Kinetic Characterization of 83753-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83753-4-RR against Human Decorin were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy DCN Ready-To-Use IHC Kit. DCN primary antibody included.

产品信息

The immunogen of 83753-4-PBS is Decorin Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Decorin fusion protein Ag6275 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name decorin
Synonyms DCN, PG40, PG S2, DSPG2, CSCD
Calculated Molecular Weight 359 aa, 40 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC005322
Gene Symbol Decorin
Gene ID (NCBI) 1634
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP07585
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. The binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. Decorin(~ 40 kDa) also binds glycosaminoglycan chains to form big molecules.