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验证数据展示

  • WB analysis using 83753-4-RR

    WB analysis using 83753-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83753-4-RR (Decorin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83753-4-RR

    FC experiment of HepG2 using 83753-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Decorin Recombinant antibody (83753-4-RR, Clone:240781E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HEK-293 using 83753-4-RR

    FC experiment of HEK-293 using 83753-4-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Decorin Recombinant antibody (83753-4-RR, Clone:240781E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83753-4-RR

    Affinity and Kinetic Characterization of 83753-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83753-4-RR against Human Decorin were performed. The affinity constant is below 1 pM.

Decorin Recombinant antibody

Decorin Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83753-4-RR

产品说明书

CloneNo. 240781E3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

DCN, PG40, PG S2, DSPG2, CSCD

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, ROS1728 cells, mouse colon tissue
Positive FC (Intra) detected inHEK-293 cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DCN Ready-To-Use IHC Kit. DCN primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83753-4-RR targets Decorin in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Decorin fusion protein Ag6275 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 decorin
别名 DCN, PG40, PG S2, DSPG2, CSCD
计算分子量 359 aa, 40 kDa
观测分子量70 kDa
GenBank蛋白编号BC005322
基因名称 Decorin
Gene ID (NCBI) 1634
RRIDAB_3671350
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP07585
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. The binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. Decorin(~ 40 kDa) also binds glycosaminoglycan chains to form big molecules.

实验方案

Product Specific Protocols
WB protocol for Decorin antibody 83753-4-RRDownload protocol
FC protocol for Decorin antibody 83753-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols