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Conjugate
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Decorin Recombinant antibody

Decorin Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240781E3

Cat No : 83753-4-RR

Print datasheet

Synonyms

DCN, PG40, PG S2, DSPG2, CSCD

验证数据展示

  • WB analysis using 83753-4-RR

    WB analysis using 83753-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83753-4-RR (Decorin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83753-4-RR

    FC experiment of HepG2 using 83753-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Decorin Recombinant antibody (83753-4-RR, Clone:240781E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HEK-293 using 83753-4-RR

    FC experiment of HEK-293 using 83753-4-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Decorin Recombinant antibody (83753-4-RR, Clone:240781E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83753-4-RR

    Affinity and Kinetic Characterization of 83753-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83753-4-RR against Human Decorin were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inMCF-7 cells, ROS1728 cells, mouse colon tissue
Positive FC (Intra) detected inHEK-293 cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DCN Ready-To-Use IHC Kit. DCN primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83753-4-RR is Decorin Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Decorin fusion protein Ag6275 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name decorin
Synonyms DCN, PG40, PG S2, DSPG2, CSCD
Calculated Molecular Weight 359 aa, 40 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC005322
Gene Symbol Decorin
Gene ID (NCBI) 1634
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP07585
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. The binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. Decorin(~ 40 kDa) also binds glycosaminoglycan chains to form big molecules.

实验方案

Product Specific Protocols
WB protocol for Decorin antibody 83753-4-RRDownload protocol
FC protocol for Decorin antibody 83753-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols