验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, ROS1728 cells, mouse colon tissue |
Positive FC (Intra) detected in | HEK-293 cells, HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83753-4-RR targets Decorin in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, FC (Intra), ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | Decorin fusion protein Ag6275 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | decorin |
Synonyms | DCN, PG40, PG S2, DSPG2, CSCD |
Calculated Molecular Weight | 359 aa, 40 kDa |
Observed Molecular Weight | 70 kDa |
GenBank Accession Number | BC005322 |
Gene Symbol | Decorin |
Gene ID (NCBI) | 1634 |
RRID | AB_3671350 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P07585 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. The binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. Decorin(~ 40 kDa) also binds glycosaminoglycan chains to form big molecules.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Decorin antibody 83753-4-RR | Download protocol |
FC protocol for Decorin antibody 83753-4-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |