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DCP1A Monoclonal antibody

DCP1A Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D11E2

Cat no : 60494-1-Ig

Print datasheet

Synonyms

Nbla00360, mRNA-decapping enzyme 1A, mRNA decapping enzyme 1A, HSA275986, EC:3.6.1.62

验证数据展示

  • WB analysis using 60494-1-Ig

    WB analysis using 60494-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60494-1-Ig (DCP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 60494-1-Ig

    WB analysis using 60494-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60494-1-Ig (DCP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inLoVo cells, rat brain tissue, HCT 116 cells, HEK-293 cells, Jurkat cells, MOLT-4 cells, Raji cells, mouse brain tissue, pig brain tissue
Planning an IHC experiment? We recommend our IHCeasy DCP1A Ready-To-Use IHC Kit. DCP1A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60494-1-Ig targets DCP1A in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen DCP1A fusion protein Ag18706 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name DCP1 decapping enzyme homolog A (S. cerevisiae)
Synonyms Nbla00360, mRNA-decapping enzyme 1A, mRNA decapping enzyme 1A, HSA275986, EC:3.6.1.62
Calculated Molecular Weight 582 aa, 63 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC007439
Gene Symbol DCP1A
Gene ID (NCBI) 55802
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NPI6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCP1 decapping enzyme homolog A (S. cerevisiae), known as mRNA-decapping enzyme 1A (DCP1A),is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. This antibody specifically react with the 70kd DCP1A protein.

实验方案

Product Specific Protocols
WB protocol for DCP1A antibody 60494-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols