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验证数据展示

  • WB analysis using 60494-1-Ig

    WB analysis using 60494-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60494-1-Ig (DCP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 60494-1-Ig

    WB analysis using 60494-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60494-1-Ig (DCP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

DCP1A Monoclonal antibody

DCP1A Monoclonal Antibody for WB, ELISA
Cat No. 60494-1-Ig

产品说明书

CloneNo. 2D11E2

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, ELISA

别名

Nbla00360, mRNA-decapping enzyme 1A, mRNA decapping enzyme 1A, HSA275986, EC:3.6.1.62

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLoVo cells, rat brain tissue, HCT 116 cells, HEK-293 cells, Jurkat cells, MOLT-4 cells, Raji cells, mouse brain tissue, pig brain tissue
Planning an IHC experiment? We recommend our IHCeasy DCP1A Ready-To-Use IHC Kit. DCP1A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60494-1-Ig targets DCP1A in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 DCP1A fusion protein Ag18706 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 DCP1 decapping enzyme homolog A (S. cerevisiae)
别名 Nbla00360, mRNA-decapping enzyme 1A, mRNA decapping enzyme 1A, HSA275986, EC:3.6.1.62
计算分子量 582 aa, 63 kDa
观测分子量70 kDa
GenBank蛋白编号BC007439
基因名称 DCP1A
Gene ID (NCBI) 55802
RRIDAB_3670263
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NPI6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCP1 decapping enzyme homolog A (S. cerevisiae), known as mRNA-decapping enzyme 1A (DCP1A),is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. This antibody specifically react with the 70kd DCP1A protein.

实验方案

Product Specific Protocols
WB protocol for DCP1A antibody 60494-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols