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验证数据展示

  • IF Staining of HepG2 using CL488-22373

    IF Staining of HepG2 using CL488-22373

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 DCP1A antibody (CL488-22373) at dilution of 1:100.

  • FC experiment of HeLa using CL488-22373

    FC experiment of HeLa using CL488-22373

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human DCP1A (CL488-22373) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated DCP1A Polyclonal antibody

DCP1A Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-22373

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Smad4-interacting transcriptional co-activator, Nbla00360, mRNA-decapping enzyme 1A, mRNA decapping enzyme 1A, HSA275986

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DCP1A Ready-To-Use IHC Kit. DCP1A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 22373-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-22373 targets DCP1A in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 DCP1A fusion protein Ag18027 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DCP1 decapping enzyme homolog A (S. cerevisiae)
别名 Smad4-interacting transcriptional co-activator, Nbla00360, mRNA-decapping enzyme 1A, mRNA decapping enzyme 1A, HSA275986
计算分子量 582 aa, 63 kDa
观测分子量 70 kDa
GenBank蛋白编号BC007439
基因名称 DCP1A
Gene ID (NCBI) 55802
RRIDAB_3084068
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NPI6
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCP1 decapping enzyme homolog A (S. cerevisiae), known as mRNA-decapping enzyme 1A (DCP1A),is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. This antibody specifically react with the 70kd DCP1A protein.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DCP1A antibody CL488-22373Download protocol
FC protocol for CL Plus 488 DCP1A antibody CL488-22373Download protocol
Standard Protocols
Click here to view our Standard Protocols