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验证数据展示

  • WB analysis using 67311-1-Ig

    WB analysis using 67311-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67311-1-Ig (DCPS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67311-1-Ig

    WB analysis of HeLa using 67311-1-Ig

    WB result of DCPS antibody (67311-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCPS transfected HeLa cells.

  • IHC staining of human breast cancer using 67311-1-Ig

    IHC staining of human breast cancer using 67311-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67311-1-Ig (DCPS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DCPS antibody included
  • IHC staining of human breast cancer using 67311-1-Ig

    IHC staining of human breast cancer using 67311-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67311-1-Ig (DCPS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DCPS antibody included
  • IF Staining of human liver cancer using 67311-1-Ig

    IF Staining of human liver cancer using 67311-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using DCPS antibody (67311-1-Ig, Clone: 1B2E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

DCPS Monoclonal antibody

DCPS Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67311-1-Ig

产品说明书

CloneNo. 1B2E4

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat

应用

WB, IHC, IF-P, ELISA

别名

DCPS, DCS 1, DCS1, decapping enzyme, scavenger, HINT 5, HINT5, HSL1, HSPC015

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, rat spleen tissue, mouse brain tissue, HepG2 cells, K-562 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue
Planning an IHC experiment? We recommend our IHCeasy DCPS Ready-To-Use IHC Kit. DCPS primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67311-1-Ig targets DCPS in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 DCPS fusion protein Ag8872 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 decapping enzyme, scavenger
别名 DCPS, DCS 1, DCS1, decapping enzyme, scavenger, HINT 5, HINT5, HSL1, HSPC015
计算分子量 337 aa, 39 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC014532
基因名称 DCPS
Gene ID (NCBI) 28960
RRIDAB_2882571
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96C86
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DcpS, which is a member of the histidine triad (HIT) superfamily of pyrophosphatases, is the first HIT protein with a defined biological function and identifies the HIT motif as a new mRNA decapping domain. DcpS can encode a 38-40 kDa protein, and has the potential to form a homodimer of 80 kDa(PMID: 12198172).

实验方案

Product Specific Protocols
WB protocol for DCPS antibody 67311-1-IgDownload protocol
IHC protocol for DCPS antibody 67311-1-IgDownload protocol
IF protocol for DCPS antibody 67311-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols