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验证数据展示

  • WB analysis using 67311-1-Ig (same clone as 67311-1-PBS)

    WB analysis using 67311-1-Ig (same clone as 67311-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67311-1-Ig (DCPS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67311-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67311-1-Ig (same clone as 67311-1-PBS)

    WB analysis of HeLa using 67311-1-Ig (same clone as 67311-1-PBS)

    WB result of DCPS antibody (67311-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCPS transfected HeLa cells. This data was developed using the same antibody clone with 67311-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67311-1-Ig (same clone as 67311-1-PBS)

    IHC staining of human breast cancer using 67311-1-Ig (same clone as 67311-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67311-1-Ig (DCPS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67311-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DCPS antibody included
  • IHC staining of human breast cancer using 67311-1-Ig (same clone as 67311-1-PBS)

    IHC staining of human breast cancer using 67311-1-Ig (same clone as 67311-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67311-1-Ig (DCPS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67311-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DCPS antibody included
  • IF Staining of human liver cancer using 67311-1-Ig (same clone as 67311-1-PBS)

    IF Staining of human liver cancer using 67311-1-Ig (same clone as 67311-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using DCPS antibody (67311-1-Ig, Clone: 1B2E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67311-1-PBS in a different storage buffer formulation.

DCPS Monoclonal antibody, PBS Only

DCPS Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67311-1-PBS

产品说明书

CloneNo. 1B2E4

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

DCPS, DCS 1, DCS1, decapping enzyme, scavenger, HINT 5, HINT5, HSL1, HSPC015

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DCPS Ready-To-Use IHC Kit. DCPS primary antibody included.

产品信息

67311-1-PBS targets DCPS in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原

CatNo: Ag8872

Product name: Recombinant human DCPS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-337 aa of BC014532

Sequence: MADAAPQLGKRKRELDVEEAHAASTEEKEAGVGNGTCAPVRLPFSGFRLQKVLRESARDKIIFLHGKVNEASEDGDGEDAVVILEKTPFQVEQVAQLLTGSPELQLQFSNDIYSTYHLFPPRQLNDVKTTVVYPATEKHLQKYLRQDLRLIRETGDDYRNITLPHLESQSLSIQWVYNILDKKAEADRIVFENPDPSDGFVLIPDLKWNQQQLDDLYLIAICHRRGIRSLRDLTPEHLPLLRNILHQGQEAILQRYRMKGDHLRVYLHYLPSYYHLHVHFTALGFEAPGSGVERAHLLAEVIENLECDPRHYQQRTLTFALRADDPLLKLLQEAQQS

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 decapping enzyme, scavenger
别名 DCPS, DCS 1, DCS1, decapping enzyme, scavenger, HINT 5, HINT5, HSL1, HSPC015
计算分子量 337 aa, 39 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC014532
基因名称 DCPS
Gene ID (NCBI) 28960
RRIDAB_2882571
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96C86
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DcpS, which is a member of the histidine triad (HIT) superfamily of pyrophosphatases, is the first HIT protein with a defined biological function and identifies the HIT motif as a new mRNA decapping domain. DcpS can encode a 38-40 kDa protein, and has the potential to form a homodimer of 80 kDa(PMID: 12198172).

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