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DCT Monoclonal antibody

DCT Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Pig

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B3D4

Cat No : 68114-1-Ig

Print datasheet

Synonyms

DCT, DT, L dopachrome Delta isomerase, L dopachrome tautomerase, TRP 2, TRP2, Tyrosinase related protein 2, TYRP2

验证数据展示

  • WB analysis of A375 using 68114-1-Ig

    WB analysis of A375 using 68114-1-Ig

    A375 cells were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 68114-1-Ig

    IF Staining of A549 using 68114-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using DCT antibody (68114-1-Ig, Clone: 1B3D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inA375 cells, HL-60 cells, pig skin tissue, mouse skin tissue, mouse testis tissue
Positive IF detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68114-1-Ig targets DCT in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Pig samples.

Tested Applications IF/ICC, WB, ELISA Application Description
Tested Reactivity Human, Mouse, Pig
Immunogen DCT fusion protein Ag26165 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)
Synonyms DCT, DT, L dopachrome Delta isomerase, L dopachrome tautomerase, TRP 2, TRP2, Tyrosinase related protein 2, TYRP2
Calculated Molecular Weight 519 aa, 59 kDa
Observed Molecular Weight55-63 kDa
GenBank Accession NumberBC028311
Gene Symbol DCT
Gene ID (NCBI) 1638
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP40126
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCT(L-dopachrome Delta-isomerase) is also named as TYRP2,TRP2 and belongs to the tyrosinase family.human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region(PMID:8206391).

实验方案

Product Specific Protocols
WB protocol for DCT antibody 68114-1-IgDownload protocol
IF protocol for DCT antibody 68114-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols