验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A549 cells |
For other applications, we recommend the unconjugated version of this antibody, 68114-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68114 targets DCT in IF/ICC applications and shows reactivity with human, mouse, pig samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, pig |
| 免疫原 | DCT fusion protein Ag26165 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2) |
| 别名 | TRP 2, L-dopachrome tautomerase, L-dopachrome Delta-isomerase, EC:5.3.3.12, DT |
| 计算分子量 | 519 aa, 59 kDa |
| 观测分子量 | 55-63 kDa |
| GenBank蛋白编号 | BC028311 |
| 基因名称 | DCT |
| Gene ID (NCBI) | 1638 |
| RRID | AB_3672970 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P40126 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DCT(L-dopachrome Delta-isomerase) is also named as TYRP2,TRP2 and belongs to the tyrosinase family.human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region(PMID:8206391).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 DCT antibody CL488-68114 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |