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DCTD Polyclonal antibody

DCTD Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16784-1-AP

Print datasheet

Synonyms

dCMP deaminase, DCTD, Deoxycytidylate deaminase

验证数据展示

  • WB analysis of human brain using 16784-1-AP

    WB analysis of human brain using 16784-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16784-1-AP (DCTD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16784-1-AP

    IF Staining of HepG2 using 16784-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DCTD antibody (16784-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inhuman brain tissue, PC-3 cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16784-1-AP targets DCTD in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DCTD fusion protein Ag10216 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dCMP deaminase
Synonyms dCMP deaminase, DCTD, Deoxycytidylate deaminase
Calculated Molecular Weight 178 aa, 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC001286
Gene Symbol DCTD
Gene ID (NCBI) 1635
RRIDAB_2261528
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32321
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCTD(Deoxycytidylate deaminase) is also named as dCMP deaminase and belongs to the cytidine and deoxycytidylate deaminase family. It catalyzes the deamination of dCMP to dUMP, thus providing the nucleotide substrate for thymidylate synthase. Control of deaminase activity at this juncture in deoxyribonucleotide metabolism is determined by the ratio of dCTP to dTTP in the cell, since the enzyme is allosterically activated by dCTP and inhibited by dTTP(PMID:7685356). It has 2 isoforms produced by alternative splicing and can exsit as a dimer(pMID:8798492).

实验方案

Product Specific Protocols
WB protocol for DCTD antibody 16784-1-APDownload protocol
IF protocol for DCTD antibody 16784-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Cancer

Adult T-cell leukemia-lymphoma acquires resistance to DNA demethylating agents through dysregulation of enzymes involved in pyrimidine metabolism.

Authors - Nao Yoshida-Sakai
View Article