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验证数据展示

  • IF Staining of HepG2 using CL488-16784

    IF Staining of HepG2 using CL488-16784

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 DCTD antibody (CL488-16784) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-16784

    FC experiment of HepG2 using CL488-16784

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 DCTD Polyclonal Antibody (CL488-16784)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated DCTD Polyclonal antibody

DCTD Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-16784

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

EC:3.5.4.12, Deoxycytidylate deaminase, dCMP deaminase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 16784-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16784 targets DCTD in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 DCTD fusion protein Ag10216 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dCMP deaminase
别名 EC:3.5.4.12, Deoxycytidylate deaminase, dCMP deaminase
计算分子量 178 aa, 20 kDa
观测分子量 20 kDa
GenBank蛋白编号BC001286
基因名称 DCTD
Gene ID (NCBI) 1635
RRIDAB_3672661
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP32321
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCTD(Deoxycytidylate deaminase) is also named as dCMP deaminase and belongs to the cytidine and deoxycytidylate deaminase family. It catalyzes the deamination of dCMP to dUMP, thus providing the nucleotide substrate for thymidylate synthase. Control of deaminase activity at this juncture in deoxyribonucleotide metabolism is determined by the ratio of dCTP to dTTP in the cell, since the enzyme is allosterically activated by dCTP and inhibited by dTTP(PMID:7685356). It has 2 isoforms produced by alternative splicing and can exsit as a dimer(pMID:8798492).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DCTD antibody CL488-16784Download protocol
FC protocol for CL Plus 488 DCTD antibody CL488-16784Download protocol
Standard Protocols
Click here to view our Standard Protocols