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CoraLite® Plus 488-conjugated DCTD Polyclonal antibody

DCTD Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-16784

Print datasheet

Synonyms

EC:3.5.4.12, Deoxycytidylate deaminase, dCMP deaminase

验证数据展示

  • IF Staining of HepG2 using CL488-16784

    IF Staining of HepG2 using CL488-16784

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 DCTD antibody (CL488-16784) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-16784

    FC experiment of HepG2 using CL488-16784

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 DCTD Polyclonal Antibody (CL488-16784)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 16784-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16784 targets DCTD in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen DCTD fusion protein Ag10216 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dCMP deaminase
Synonyms EC:3.5.4.12, Deoxycytidylate deaminase, dCMP deaminase
Calculated Molecular Weight 178 aa, 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC001286
Gene Symbol DCTD
Gene ID (NCBI) 1635
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32321
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCTD(Deoxycytidylate deaminase) is also named as dCMP deaminase and belongs to the cytidine and deoxycytidylate deaminase family. It catalyzes the deamination of dCMP to dUMP, thus providing the nucleotide substrate for thymidylate synthase. Control of deaminase activity at this juncture in deoxyribonucleotide metabolism is determined by the ratio of dCTP to dTTP in the cell, since the enzyme is allosterically activated by dCTP and inhibited by dTTP(PMID:7685356). It has 2 isoforms produced by alternative splicing and can exsit as a dimer(pMID:8798492).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DCTD antibody CL488-16784Download protocol
FC protocol for CL Plus 488 DCTD antibody CL488-16784Download protocol
Standard Protocols
Click here to view our Standard Protocols