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DCX Polyclonal antibody, PBS Only

DCX Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13925-1-PBS

Print datasheet

Synonyms

Lis-X, Lissencephalin-X, Doublin, doublecortin

验证数据展示

  • WB analysis using 13925-1-AP (same clone as 13925-1-PBS)

    WB analysis using 13925-1-AP (same clone as 13925-1-PBS)

    Fetal human brain lysates were subjected to SDS PAGE followed by western blot with 13925-1-AP (DCX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13925-1-PBS in a different storage buffer formulation.

  • WB analysis using 13925-1-AP (same clone as 13925-1-PBS)

    WB analysis using 13925-1-AP (same clone as 13925-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13925-1-AP (DCX antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13925-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 13925-1-AP (same clone as 13925-1-PBS)

    IF Staining of SH-SY5Y using 13925-1-AP (same clone as 13925-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DCX antibody (13925-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13925-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 13925-1-AP (same clone as 13925-1-PBS)

    IHC staining of human gliomas using 13925-1-AP (same clone as 13925-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13925-1-AP (DCX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13925-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 13925-1-AP (same clone as 13925-1-PBS)

    IHC staining of rat brain using 13925-1-AP (same clone as 13925-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13925-1-AP (DCX antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13925-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 13925-1-AP (same clone as 13925-1-PBS)

    IHC staining of mouse brain using 13925-1-AP (same clone as 13925-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13925-1-AP (DCX antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13925-1-PBS in a different storage buffer formulation.

产品信息

13925-1-PBS targets DCX in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DCX fusion protein Ag4945 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name doublecortin
Synonyms Lis-X, Lissencephalin-X, Doublin, doublecortin
Calculated Molecular Weight 402 aa, 45 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC027925
Gene Symbol DCX
Gene ID (NCBI) 1641
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43602
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DCX (Doublecortin) is a microtubule-associated protein that is essential for normal brain development. DCX is expressed in migrating neurons throughout the central and peripheral nervous system during embryonic and postnatal development. Anti-DCX antibodies are widely used for detection of neurogenesis. It's also a common neuron marker since its expression is specific to neurons.