DOPA decarboxylase Polyclonal antibody

DOPA decarboxylase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Publications

Cat no : 10166-1-AP

Print datasheet

Synonyms

DDC, EC:4.1.1.28, Aromatic-L-amino-acid decarboxylase, AADC

验证数据展示

WB analysis using 10166-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 10166-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of mouse kidney using 10166-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with DDC antibody included
IHC staining of mouse kidney using 10166-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with DDC antibody included
IHC staining of rat kidney using 10166-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with DDC antibody included
IHC staining of rat kidney using 10166-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with DDC antibody included
IP experiment of mouse brain using 10166-1-AP
IP result of anti-DOPA decarboxylase (IP:10166-1-AP, 4ug; Detection:10166-1-AP 1:800) with mouse brain tissue lysate 4000ug.
IF Staining of SH-SY5Y using 10166-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DOPA decarboxylase antibody (10166-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 10166-1-AP

Size

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经过测试的应用

Positive WB detected in	SH-SY5Y cells, mouse kidney tissue, mouse brain tissue, rat kidney tissue, PC-12 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse kidney tissue, human liver cancer tissue, rat kidney tissue, rat small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	SH-SY5Y cells

Planning an IHC experiment? We recommend our IHCeasy DDC Ready-To-Use IHC Kit. DDC primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 5 publications below
IHC	See 7 publications below
IF	See 2 publications below

产品信息

10166-1-AP targets DOPA decarboxylase in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, IP, ELISA Application Description
Cited Applications	WB, IHC, IF
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Immunogen	DOPA decarboxylase fusion protein Ag0219 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	dopa decarboxylase (aromatic L-amino acid decarboxylase)
Synonyms	DDC, EC:4.1.1.28, Aromatic-L-amino-acid decarboxylase, AADC
Calculated Molecular Weight	54 kDa
Observed Molecular Weight	48-50 kDa
GenBank Accession Number	BC008366
Gene Symbol	DOPA decarboxylase
Gene ID (NCBI)	1644
RRID	AB_2088937
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P20711
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Aromatic-L-amino-acid decarboxylase belongs to the pyridoxal-dependent aminotransferase superfamily.DDC catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.DDC is the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD).Researches showed that Ddc is only one of the enzymes in the biosynthetic pathways for bioamines and catecholamines.

实验方案

Product Specific Protocols
WB protocol for DOPA decarboxylase antibody 10166-1-AP	Download protocol
IHC protocol for DOPA decarboxylase antibody 10166-1-AP	Download protocol
IF protocol for DOPA decarboxylase antibody 10166-1-AP	Download protocol
IP protocol for DOPA decarboxylase antibody 10166-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	IF	Nature Reversing a model of Parkinson's disease with in situ converted nigral neurons. Authors - Hao Qian View Article
human,mouse	WB,IHC	Cell Signal Profile of 5-HT2A receptor involved in signaling cascades associated to intracellular inflammation and apoptosis in hepatocytes and its role in carbon tetrachloride-induced hepatotoxicity Authors - Yuxin Zhang View Article
human	WB	Dis Model Mech A Matrigel-based 3D construct of SH-SY5Y cells models the α-synuclein pathologies of Parkinson's disease. Authors - Zhao-Feng Li View Article
mouse	IHC,IF	Toxicol Appl Pharmacol PMID: 21703292 Authors - Fox Donald A DA View Article
human	WB	J Transl Med Retinal pigment epithelial cells secrete neurotrophic factors and synthesize dopamine: possible contribution to therapeutic effects of RPE cell transplantation in Parkinson's disease. Authors - Ming Ming View Article
human,mouse	WB,IHC	Toxicol Appl Pharmacol Carbon tetrachloride induced mitochondrial division, respiratory chain damage, abnormal intracellular [H+] and apoptosis are due to the activation of 5-HT degradation system in hepatocytes. Authors - Yuxin Zhang View Article

DOPA decarboxylase Polyclonal antibody

DOPA decarboxylase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

验证数据展示

WB analysis using 10166-1-AP

WB analysis using 10166-1-AP

IHC staining of mouse kidney using 10166-1-AP

IHC staining of mouse kidney using 10166-1-AP

IHC staining of rat kidney using 10166-1-AP

IHC staining of rat kidney using 10166-1-AP

IP experiment of mouse brain using 10166-1-AP

IF Staining of SH-SY5Y using 10166-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Reversing a model of Parkinson's disease with in situ converted nigral neurons.

Profile of 5-HT2A receptor involved in signaling cascades associated to intracellular inflammation and apoptosis in hepatocytes and its role in carbon tetrachloride-induced hepatotoxicity

A Matrigel-based 3D construct of SH-SY5Y cells models the α-synuclein pathologies of Parkinson's disease.

PMID: 21703292

Retinal pigment epithelial cells secrete neurotrophic factors and synthesize dopamine: possible contribution to therapeutic effects of RPE cell transplantation in Parkinson's disease.

Carbon tetrachloride induced mitochondrial division, respiratory chain damage, abnormal intracellular [H+] and apoptosis are due to the activation of 5-HT degradation system in hepatocytes.