验证数据展示
经过测试的应用
Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-10166 targets DOPA decarboxylase/DDC in IF/ICC applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | DOPA decarboxylase/DDC fusion protein Ag0219 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | dopa decarboxylase (aromatic L-amino acid decarboxylase) |
别名 | DOPA decarboxylase, DDC, EC:4.1.1.28, Aromatic-L-amino-acid decarboxylase, AADC |
计算分子量 | 54 kDa |
观测分子量 | 48-50 kDa |
GenBank蛋白编号 | BC008366 |
基因名称 | DOPA decarboxylase |
Gene ID (NCBI) | 1644 |
RRID | AB_3672430 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P20711 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Aromatic-L-amino-acid decarboxylase belongs to the pyridoxal-dependent aminotransferase superfamily.DDC catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.DDC is the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD).Researches showed that Ddc is only one of the enzymes in the biosynthetic pathways for bioamines and catecholamines.
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 DOPA decarboxylase/DDC antibody CL488-10166 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |