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验证数据展示

  • IF Staining of SH-SY5Y using CL488-10166

    IF Staining of SH-SY5Y using CL488-10166

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 DOPA decarboxylase/DDC antibody (CL488-10166) at dilution of 1:200.

CoraLite® Plus 488-conjugated DOPA decarboxylase/DDC Polyclonal antibody

DOPA decarboxylase/DDC Polyclonal Antibody for IF/ICC
Cat No. CL488-10166

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

DOPA decarboxylase, DDC, EC:4.1.1.28, Aromatic-L-amino-acid decarboxylase, AADC

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy DDC Ready-To-Use IHC Kit. DDC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10166-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10166 targets DOPA decarboxylase/DDC in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen DOPA decarboxylase/DDC fusion protein Ag0219 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dopa decarboxylase (aromatic L-amino acid decarboxylase)
Synonyms DOPA decarboxylase, DDC, EC:4.1.1.28, Aromatic-L-amino-acid decarboxylase, AADC
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 48-50 kDa
GenBank Accession NumberBC008366
Gene Symbol DOPA decarboxylase
Gene ID (NCBI) 1644
RRIDAB_3672430
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20711
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aromatic-L-amino-acid decarboxylase belongs to the pyridoxal-dependent aminotransferase superfamily.DDC catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.DDC is the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD).Researches showed that Ddc is only one of the enzymes in the biosynthetic pathways for bioamines and catecholamines.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DOPA decarboxylase/DDC antibody CL488-10166Download protocol
Standard Protocols
Click here to view our Standard Protocols