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DDHD2 Polyclonal antibody

DDHD2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25203-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (2)

DDHD domain containing 2, DDHD2, KIAA0725, KIAA0725p, Phospholipase DDHD2, SAMWD1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inrat brain tissue, mouse lung tissue, mouse testis tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25203-1-AP targets DDHD2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 DDHD2 fusion protein Ag18373 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DDHD domain containing 2
别名 DDHD domain containing 2, DDHD2, KIAA0725, KIAA0725p, Phospholipase DDHD2, SAMWD1
计算分子量 711 aa, 81 kDa
观测分子量 80-90 kDa
GenBank蛋白编号BC010504
基因名称 DDHD2
Gene ID (NCBI) 23259
RRIDAB_2879957
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94830
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDHD2, also known as KIAA0725p, is a member of the intracellular phospholipase A1 (PLA1) protein family which comprises a group of enzymes that hydrolyze the sn-1 ester bond of phospholipids, producing 2-acyl-lysophospholipids and fatty acids. DDHD2 prefers phosphatidic acid as substrate and has a role in efficient membrane trafficking from the Golgi apparatus to the plasma membrane. The gene of DDHD2 maps to chromosome 8p11.23, and encodes a 711-amino-acid protein with a calculated molecular mass of 81 kDa. It has been reported that DDHD2 immunoblot analysis of various tissue extracts revealed two bands of 90 and 85 kDa (PMID: 11788596).

实验方案

Product Specific Protocols
WB protocol for DDHD2 antibody 25203-1-APDownload protocol
IHC protocol for DDHD2 antibody 25203-1-APDownload protocol
IF protocol for DDHD2 antibody 25203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Protein Cell

Proteomic analysis of ferroptosis pathways reveals a role of CEPT1 in suppressing ferroptosis

Authors - Xiaoguang Liu
mouse

Neuron

Rewiring Neuronal Glycerolipid Metabolism Determines the Extent of Axon Regeneration.

Authors - Chao Yang
  • KD Validated
mouseWB

J Biol Chem

The phospholipase PNPLA7 functions as a lysophosphatidylcholine hydrolase and interacts with lipid droplets through its catalytic domain.

Authors - Christoph Heier
humanWB,IHC

Cell Death Dis

Circular RNA circRUNX1 promotes papillary thyroid cancer progression and metastasis by sponging MiR-296-3p and regulating DDHD2 expression.

Authors - Junjie Chu
  • KD Validated
mouseWB,IF

J Lipid Res

Cooperative lipolytic control of neuronal triacylglycerol by spastic paraplegia-associated enzyme DDHD2 and ATGL

Authors - Peter Hofer
ratWB,IF,IP

EMBO J

The DDHD2-STXBP1 interaction mediates long-term memory via generation of saturated free fatty acids

Authors - Isaac O Akefe
  • KO Validated
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