验证数据展示
经过测试的应用
Positive IF/ICC detected in | HEK-293 cells |
Positive FC (Intra) detected in | HEK-293 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-25203 targets DDHD2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IF/ICC, FC (Intra) Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | DDHD2 fusion protein Ag18373 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | DDHD domain containing 2 |
别名 | SAM, WWE and DDHD domain-containing protein 1, Phospholipase DDHD2, KIAA0725p, KIAA0725, EC:3.1.1.- |
计算分子量 | 711 aa, 81 kDa |
观测分子量 | 80-90 kDa |
GenBank蛋白编号 | BC010504 |
基因名称 | DDHD2 |
Gene ID (NCBI) | 23259 |
RRID | AB_3672788 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O94830 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DDHD2, also known as KIAA0725p, is a member of the intracellular phospholipase A1 (PLA1) protein family which comprises a group of enzymes that hydrolyze the sn-1 ester bond of phospholipids, producing 2-acyl-lysophospholipids and fatty acids. DDHD2 prefers phosphatidic acid as substrate and has a role in efficient membrane trafficking from the Golgi apparatus to the plasma membrane. The gene of DDHD2 maps to chromosome 8p11.23, and encodes a 711-amino-acid protein with a calculated molecular mass of 81 kDa. It has been reported that DDHD2 immunoblot analysis of various tissue extracts revealed two bands of 90 and 85 kDa (PMID: 11788596).
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 DDHD2 antibody CL488-25203 | Download protocol |
FC protocol for CL Plus 488 DDHD2 antibody CL488-25203 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |