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DDX1 Monoclonal antibody

DDX1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G10G4

Cat no : 67991-1-Ig

Print datasheet

Synonyms

DBP RB, DDX1, DEAD box protein 1, UKVH5d

验证数据展示

  • WB analysis using 67991-1-Ig

    WB analysis using 67991-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67991-1-Ig (DDX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of 4T1 using 67991-1-Ig

    WB analysis of 4T1 using 67991-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67991-1-Ig (DDX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67991-1-Ig

    WB analysis of HeLa using 67991-1-Ig

    WB result of DDX1 antibody (67991-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX1 transfected HeLa cells.

经过测试的应用

Positive WB detected inA431 cells, 4T1 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy DDX1 Ready-To-Use IHC Kit. DDX1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67991-1-Ig targets DDX1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen DDX1 fusion protein Ag16774 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
Synonyms DBP RB, DDX1, DEAD box protein 1, UKVH5d
Calculated Molecular Weight 740 aa, 82 kDa
Observed Molecular Weight82 kDa
GenBank Accession NumberBC012132
Gene Symbol DDX1
Gene ID (NCBI) 1653
RRIDAB_2918740
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ92499
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX1 is a DEAD box protein, which is putative RNA helicases with a characteristic asp-glu-ala-asp (DEAD) box motif. DEAD box proteins involve in translation initiation, splicing, and ribosome and spliceosome assembly by altering RNA secondary structure. As a RNA helicase, DDX1 has a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome.

实验方案

Product Specific Protocols
WB protocol for DDX1 antibody 67991-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols