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验证数据展示

  • WB analysis using 67991-1-Ig

    WB analysis using 67991-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67991-1-Ig (DDX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of 4T1 using 67991-1-Ig

    WB analysis of 4T1 using 67991-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67991-1-Ig (DDX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67991-1-Ig

    WB analysis of HeLa using 67991-1-Ig

    WB result of DDX1 antibody (67991-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX1 transfected HeLa cells.

  • IHC staining of mouse brain using 67991-1-Ig

    IHC staining of mouse brain using 67991-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of mouse brain using 67991-1-Ig

    IHC staining of mouse brain using 67991-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of mouse lung using 67991-1-Ig

    IHC staining of mouse lung using 67991-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of mouse lung using 67991-1-Ig

    IHC staining of mouse lung using 67991-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of mouse skin using 67991-1-Ig

    IHC staining of mouse skin using 67991-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of mouse skin using 67991-1-Ig

    IHC staining of mouse skin using 67991-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of rat skin using 67991-1-Ig

    IHC staining of rat skin using 67991-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of rat skin using 67991-1-Ig

    IHC staining of rat skin using 67991-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDX1 antibody included
  • Featured Product
  • KD/KO验证

DDX1 Monoclonal antibody

DDX1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67991-1-Ig

产品说明书

CloneNo. 1G10G4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

DEAD box protein retinoblastoma, DEAD box protein 1, DBP-RB, DBP RB, ATP-dependent RNA helicase DDX1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, 4T1 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse brain tissue, mouse lung tissue, mouse skin tissue, rat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DDX1 Ready-To-Use IHC Kit. DDX1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67991-1-Ig targets DDX1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DDX1 fusion protein Ag16774 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
别名 DEAD box protein retinoblastoma, DEAD box protein 1, DBP-RB, DBP RB, ATP-dependent RNA helicase DDX1
计算分子量 740 aa, 82 kDa
观测分子量82 kDa
GenBank蛋白编号BC012132
基因名称 DDX1
Gene ID (NCBI) 1653
RRIDAB_2918740
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ92499
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX1 is a DEAD box protein, which is putative RNA helicases with a characteristic asp-glu-ala-asp (DEAD) box motif. DEAD box proteins involve in translation initiation, splicing, and ribosome and spliceosome assembly by altering RNA secondary structure. As a RNA helicase, DDX1 has a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome.

实验方案

Product Specific Protocols
WB protocol for DDX1 antibody 67991-1-IgDownload protocol
IHC protocol for DDX1 antibody 67991-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols