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验证数据展示

  • WB analysis using 67991-1-Ig (same clone as 67991-1-PBS)

    WB analysis using 67991-1-Ig (same clone as 67991-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67991-1-Ig (DDX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

  • WB analysis of 4T1 using 67991-1-Ig (same clone as 67991-1-PBS)

    WB analysis of 4T1 using 67991-1-Ig (same clone as 67991-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67991-1-Ig (DDX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67991-1-Ig (same clone as 67991-1-PBS)

    WB analysis of HeLa using 67991-1-Ig (same clone as 67991-1-PBS)

    WB result of DDX1 antibody (67991-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX1 transfected HeLa cells. This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67991-1-Ig (same clone as 67991-1-PBS)

    IHC staining of mouse brain using 67991-1-Ig (same clone as 67991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of mouse brain using 67991-1-Ig (same clone as 67991-1-PBS)

    IHC staining of mouse brain using 67991-1-Ig (same clone as 67991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of mouse lung using 67991-1-Ig (same clone as 67991-1-PBS)

    IHC staining of mouse lung using 67991-1-Ig (same clone as 67991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of mouse lung using 67991-1-Ig (same clone as 67991-1-PBS)

    IHC staining of mouse lung using 67991-1-Ig (same clone as 67991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of mouse skin using 67991-1-Ig (same clone as 67991-1-PBS)

    IHC staining of mouse skin using 67991-1-Ig (same clone as 67991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of mouse skin using 67991-1-Ig (same clone as 67991-1-PBS)

    IHC staining of mouse skin using 67991-1-Ig (same clone as 67991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of rat skin using 67991-1-Ig (same clone as 67991-1-PBS)

    IHC staining of rat skin using 67991-1-Ig (same clone as 67991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of rat skin using 67991-1-Ig (same clone as 67991-1-PBS)

    IHC staining of rat skin using 67991-1-Ig (same clone as 67991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDX1 antibody included
  • Featured Product
  • KD/KO验证

DDX1 Monoclonal antibody, PBS Only

DDX1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67991-1-PBS

产品说明书

CloneNo. 1G10G4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

DEAD box protein retinoblastoma, DEAD box protein 1, DBP-RB, DBP RB, ATP-dependent RNA helicase DDX1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy DDX1 Ready-To-Use IHC Kit. DDX1 primary antibody included.

产品信息

67991-1-PBS targets DDX1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DDX1 fusion protein Ag16774 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
别名 DEAD box protein retinoblastoma, DEAD box protein 1, DBP-RB, DBP RB, ATP-dependent RNA helicase DDX1
计算分子量 740 aa, 82 kDa
观测分子量82 kDa
GenBank蛋白编号BC012132
基因名称 DDX1
Gene ID (NCBI) 1653
RRIDAB_2918740
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ92499
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX1 is a DEAD box protein, which is putative RNA helicases with a characteristic asp-glu-ala-asp (DEAD) box motif. DEAD box proteins involve in translation initiation, splicing, and ribosome and spliceosome assembly by altering RNA secondary structure. As a RNA helicase, DDX1 has a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome.