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验证数据展示

  • WB analysis using 83106-1-RR

    WB analysis using 83106-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83106-1-RR (DDX19A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of U2OS using 83106-1-RR

    FC experiment of U2OS using 83106-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug DDX19A Recombinant antibody (83106-1-RR, Clone:230232B12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83106-1-RR

    Affinity and Kinetic Characterization of 83106-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83106-1-RR against Human DDX19A were performed. The affinity constant is below 1 pM.

DDX19A Recombinant antibody

DDX19A Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83106-1-RR

产品说明书

CloneNo. 230232B12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

EC:3.6.4.13, DDX19-like protein, DDX19L, DDX19 DDX19L, ATP-dependent RNA helicase DDX19A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 750
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, HepG2 cells, mouse thymus tissue
Positive FC (Intra) detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83106-1-RR targets DDX19A in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 DDX19A fusion protein Ag7362 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-As) box polypeptide 19A
别名 EC:3.6.4.13, DDX19-like protein, DDX19L, DDX19 DDX19L, ATP-dependent RNA helicase DDX19A
计算分子量 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC005162
基因名称 DDX19A
Gene ID (NCBI) 55308
RRIDAB_3670818
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NUU7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX19A, also known as DDX19L, belongs to the DEAD-box helicase family. DDX19A was identified as a novel cytosolic RNA sensor that could activate the NLRP3 inflammasome during virus infection. In addition, DDX19A was proven to be associated with NADPH oxidase 1 (NOX1)-mediated oxidative stress in tumor necrosis factor (TNF)-α-induced A549 cell (PMID: 33968728). It may represent biomarkers of metastasis and novel therapeutic targets in CSCC patients.

实验方案

Product Specific Protocols
WB protocol for DDX19A antibody 83106-1-RRDownload protocol
IHC protocol for DDX19A antibody 83106-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols