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CoraLite® Plus 750-conjugated DDX19A Recombinant antibody

DDX19A Uni-rAbTM Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230232B12

Cat no : CL750-83106

Print datasheet

Synonyms

EC:3.6.4.13, DDX19-like protein, DDX19L, DDX19 DDX19L, ATP-dependent RNA helicase DDX19A

验证数据展示

  • WB analysis using CL750-83106

    WB analysis using CL750-83106

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-83106 (DDX19A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • FC experiment of U2OS using CL750-83106

    FC experiment of U2OS using CL750-83106

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated DDX19A Recombinant antibody (CL750-83106, Clone:230232B12)(red), or 0.4 ug Isotype Control (CL750-3000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, K-562 cells
Positive FC (Intra) detected inU2OS cells
For other applications, we recommend the unconjugated version of this antibody, 83106-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-83106 targets DDX19A in WB applications and shows reactivity with human, mouse samples.

Tested Applications WB Application Description
Tested Reactivity human, mouse
Immunogen DDX19A fusion protein Ag7362 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name DEAD (Asp-Glu-Ala-As) box polypeptide 19A
Synonyms EC:3.6.4.13, DDX19-like protein, DDX19L, DDX19 DDX19L, ATP-dependent RNA helicase DDX19A
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC005162
Gene Symbol DDX19A
Gene ID (NCBI) 55308
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NUU7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX19A, also known as DDX19L, belongs to the DEAD-box helicase family. DDX19A was identified as a novel cytosolic RNA sensor that could activate the NLRP3 inflammasome during virus infection. In addition, DDX19A was proven to be associated with NADPH oxidase 1 (NOX1)-mediated oxidative stress in tumor necrosis factor (TNF)-α-induced A549 cell (PMID: 33968728). It may represent biomarkers of metastasis and novel therapeutic targets in CSCC patients.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 DDX19A antibody CL750-83106Download protocol
Standard Protocols
Click here to view our Standard Protocols