• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 750-conjugated DDX19A Recombinant antibody

DDX19A Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230232B12

Cat No : CL750-83106

Print datasheet

Synonyms

DDX19 DDX19L, DDX19 like protein, DDX19A, DDX19L, DEAD box protein 19A, DKFZp686C21137, FLJ11126

验证数据展示

  • WB analysis using CL750-83106

    WB analysis using CL750-83106

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-83106 (DDX19A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, K-562 cells
For other applications, we recommend the unconjugated version of this antibody, 83106-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-83106 targets DDX19A in WB applications and shows reactivity with Human, mouse samples.

Tested Applications WB Application Description
Tested Reactivity Human, mouse
Immunogen DDX19A fusion protein Ag7362 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name DEAD (Asp-Glu-Ala-As) box polypeptide 19A
Synonyms DDX19 DDX19L, DDX19 like protein, DDX19A, DDX19L, DEAD box protein 19A, DKFZp686C21137, FLJ11126
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC005162
Gene Symbol DDX19A
Gene ID (NCBI) 55308
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NUU7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX19A, also known as DDX19L, belongs to the DEAD-box helicase family. DDX19A was identified as a novel cytosolic RNA sensor that could activate the NLRP3 inflammasome during virus infection. In addition, DDX19A was proven to be associated with NADPH oxidase 1 (NOX1)-mediated oxidative stress in tumor necrosis factor (TNF)-α-induced A549 cell (PMID: 33968728). It may represent biomarkers of metastasis and novel therapeutic targets in CSCC patients.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 DDX19A antibody CL750-83106Download protocol
Standard Protocols
Click here to view our Standard Protocols