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DDX3 Monoclonal antibody, PBS Only

DDX3 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D3A1

Cat no : 67915-1-PBS

Print datasheet

Synonyms

DDX3X, DDX14, DBX, CAP-Rf, ATP-dependent RNA helicase DDX3X

验证数据展示

  • WB analysis using 67915-1-Ig (same clone as 67915-1-PBS)

    WB analysis using 67915-1-Ig (same clone as 67915-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67915-1-Ig (DDX3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67915-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 67915-1-Ig (same clone as 67915-1-PBS)

    WB analysis of HepG2 using 67915-1-Ig (same clone as 67915-1-PBS)

    WB result of DDX3 antibody (67915-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX3 transfected HepG2 cells. This data was developed using the same antibody clone with 67915-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67915-1-Ig (same clone as 67915-1-PBS)

    IHC staining of human breast cancer using 67915-1-Ig (same clone as 67915-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67915-1-Ig (DDX3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67915-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDX3 antibody included
  • IHC staining of human breast cancer using 67915-1-Ig (same clone as 67915-1-PBS)

    IHC staining of human breast cancer using 67915-1-Ig (same clone as 67915-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67915-1-Ig (DDX3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67915-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDX3 antibody included
  • FC experiment of HepG2 using 67915-1-Ig (same clone as 67915-1-PBS)

    FC experiment of HepG2 using 67915-1-Ig (same clone as 67915-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human DDX3 (67915-1-Ig, Clone:1D3A1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67915-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy DDX3 Ready-To-Use IHC Kit. DDX3 primary antibody included.

产品信息

67915-1-PBS targets DDX3 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen DDX3 fusion protein Ag29972 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
Synonyms DDX3X, DDX14, DBX, CAP-Rf, ATP-dependent RNA helicase DDX3X
Calculated Molecular Weight 73 kDa
Observed Molecular Weight71-73 kDa
GenBank Accession NumberBC011819
Gene Symbol DDX3
Gene ID (NCBI) 1654
RRIDAB_2918669
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00571
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX3X, also named DBX, DDX3, and HLP2, belongs to the DEAD-box helicase family and DDX3/DED1 subfamily. It is ATP-dependent RNA helicase. DDX3X acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It is also involved in HIV-1 replication. DDX3X interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. 73kd is the target band. 60-65kd is some other isoform. This antibody can bind both DDX3X and DDX3Y for the close sequences.