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验证数据展示

  • FC experiment of HepG2 using CL488-67915

    FC experiment of HepG2 using CL488-67915

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DDX3 (CL488-67915, Clone:1D3A1) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated DDX3 Monoclonal antibody

DDX3 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67915

产品说明书

CloneNo. 1D3A1

宿主/亚型

Mouse / IgG2b

种属反应性

human, rat

应用

FC (Intra)

别名

DDX3X, DDX14, DBX, CAP-Rf, ATP-dependent RNA helicase DDX3X

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DDX3 Ready-To-Use IHC Kit. DDX3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67915-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67915 targets DDX3 in FC (Intra) applications and shows reactivity with human, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, rat
免疫原 DDX3 fusion protein Ag29972 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
别名 DDX3X, DDX14, DBX, CAP-Rf, ATP-dependent RNA helicase DDX3X
计算分子量 73 kDa
观测分子量71-73 kDa
GenBank蛋白编号BC011819
基因名称 DDX3
Gene ID (NCBI) 1654
RRIDAB_2934591
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO00571
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX3X, also named DBX, DDX3, and HLP2, belongs to the DEAD-box helicase family and DDX3/DED1 subfamily. It is ATP-dependent RNA helicase. DDX3X acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It is also involved in HIV-1 replication. DDX3X interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. 73kd is the target band. 60-65kd is some other isoform. This antibody can bind both DDX3X and DDX3Y for the close sequences.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 DDX3 antibody CL488-67915Download protocol
Standard Protocols
Click here to view our Standard Protocols