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验证数据展示

  • FC experiment of HepG2 using CL488-67915

    FC experiment of HepG2 using CL488-67915

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DDX3 (CL488-67915, Clone:1D3A1) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DDX3 Monoclonal antibody

DDX3 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67915

Print datasheet

CloneNo. 1D3A1

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

FC (Intra)

Synonyms

DDX3X, DDX14, DBX, CAP-Rf, ATP-dependent RNA helicase DDX3X

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DDX3 Ready-To-Use IHC Kit. DDX3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67915-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67915 targets DDX3 in FC (Intra) applications and shows reactivity with human, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, rat
Immunogen DDX3 fusion protein Ag29972 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
Synonyms DDX3X, DDX14, DBX, CAP-Rf, ATP-dependent RNA helicase DDX3X
Calculated Molecular Weight 73 kDa
Observed Molecular Weight71-73 kDa
GenBank Accession NumberBC011819
Gene Symbol DDX3
Gene ID (NCBI) 1654
RRIDAB_2934591
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00571
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX3X, also named DBX, DDX3, and HLP2, belongs to the DEAD-box helicase family and DDX3/DED1 subfamily. It is ATP-dependent RNA helicase. DDX3X acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It is also involved in HIV-1 replication. DDX3X interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. 73kd is the target band. 60-65kd is some other isoform. This antibody can bind both DDX3X and DDX3Y for the close sequences.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 DDX3 antibody CL488-67915Download protocol
Standard Protocols
Click here to view our Standard Protocols