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验证数据展示

  • IF Staining of mouse testis using CL488-51042

    IF Staining of mouse testis using CL488-51042

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 DDX4,VASA antibody (CL488-51042) at dilution of 1:200.

CoraLite® Plus 488-conjugated DDX4,VASA Polyclonal antibody

DDX4,VASA Polyclonal Antibody for IF-P
Cat No. CL488-51042

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF-P

别名

DDX4, DEAD box protein 4, VASA, Vasa homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse testis tissue
For other applications, we recommend the unconjugated version of this antibody, 51042-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-51042 targets DDX4,VASA in IF-P applications and shows reactivity with human, mouse samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse
免疫原 DDX4,VASA fusion protein Ag0447 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
别名 DDX4, DEAD box protein 4, VASA, Vasa homolog
计算分子量690aa,76 kDa; 724aa,79 kDa
观测分子量 79 kDa
GenBank蛋白编号BC047455
基因名称 DDX4
Gene ID (NCBI) 54514
RRIDAB_3672850
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQI0
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DDX4,VASA antibody CL488-51042Download protocol
Standard Protocols
Click here to view our Standard Protocols