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验证数据展示

  • WB analysis of HeLa using 17591-1-AP

    WB analysis of HeLa using 17591-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17591-1-AP (DDX43 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 17591-1-AP

    IHC staining of human lung cancer using 17591-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 17591-1-AP (DDX43 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lung cancer using 17591-1-AP

    IHC staining of human lung cancer using 17591-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 17591-1-AP (DDX43 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 17591-1-AP

    IF Staining of HepG2 using 17591-1-AP

    Immunofluorescent analysis of HepG2 cells using 17591-1-AP (DDX43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

DDX43 Polyclonal antibody

DDX43 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17591-1-AP

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Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Cancer/testis antigen 13, CT13, DDX43, DEAD box protein 43, DEAD box protein HAGE, HAGE, Helical antigen

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A375 cells, HepG2 cells, PC-3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17591-1-AP targets DDX43 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DDX43 fusion protein Ag11484 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 43
Synonyms Cancer/testis antigen 13, CT13, DDX43, DEAD box protein 43, DEAD box protein HAGE, HAGE, Helical antigen
Calculated Molecular Weight 648 aa, 73 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC066938
Gene Symbol DDX43
Gene ID (NCBI) 55510
RRIDAB_2090912
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NXZ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DDX43 antibody 17591-1-APDownload protocol
IHC protocol for DDX43 antibody 17591-1-APDownload protocol
IF protocol for DDX43 antibody 17591-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Oncol Rep

Novel cancer-testis antigen expression on glioma cell lines derived from high-grade glioma patients.

Authors - Yasuto Akiyama
View Article