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验证数据展示

  • WB analysis of HeLa using 17591-1-AP

    WB analysis of HeLa using 17591-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17591-1-AP (DDX43 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 17591-1-AP

    IHC staining of human lung cancer using 17591-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 17591-1-AP (DDX43 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lung cancer using 17591-1-AP

    IHC staining of human lung cancer using 17591-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 17591-1-AP (DDX43 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 17591-1-AP

    IF Staining of HepG2 using 17591-1-AP

    Immunofluorescent analysis of HepG2 cells using 17591-1-AP (DDX43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

DDX43 Polyclonal antibody

DDX43 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17591-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Cancer/testis antigen 13, CT13, DDX43, DEAD box protein 43, DEAD box protein HAGE, HAGE, Helical antigen

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A375 cells, HepG2 cells, PC-3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17591-1-AP targets DDX43 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DDX43 fusion protein Ag11484 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 43
别名 Cancer/testis antigen 13, CT13, DDX43, DEAD box protein 43, DEAD box protein HAGE, HAGE, Helical antigen
计算分子量 648 aa, 73 kDa
观测分子量 73 kDa
GenBank蛋白编号BC066938
基因名称 DDX43
Gene ID (NCBI) 55510
RRIDAB_2090912
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NXZ2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DDX43 antibody 17591-1-APDownload protocol
IHC protocol for DDX43 antibody 17591-1-APDownload protocol
IF protocol for DDX43 antibody 17591-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Oncol Rep

Novel cancer-testis antigen expression on glioma cell lines derived from high-grade glioma patients.

Authors - Yasuto Akiyama
View Article