DDX5 Monoclonal antibody
DDX5 Monoclonal Antibody for WB, IP, IF/ICC, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (1)
Applications
WB, IP, IF/ICC, IHC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
2D3D2
验证数据展示
经过测试的应用
| Positive WB detected in | NCI-H1299 cells, A431 cells, HSC-T6 cells, HeLa cells, HEK-293 cell, HepG2 cells, HT-29 cells, NIH/3T3 cells, 4T1 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy DDX5 Ready-To-Use IHC Kit. DDX5 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF) | IF : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67025-1-Ig targets DDX5 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | WB, IP, IF/ICC, IHC, ELISA Application Description |
| Cited Applications | WB, IP, CoIP |
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | DDX5 fusion protein Ag24821 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 |
| Synonyms | DDX5, DEAD box protein 5, DKFZp434E109, DKFZp686J01190, G17P1, HELR, HLR1, HUMP68, p68, RNA helicase p68 |
| Observed Molecular Weight | 69 kDa |
| GenBank Accession Number | NM_004396 |
| Gene Symbol | DDX5 |
| Gene ID (NCBI) | 1655 |
| RRID | AB_2882340 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P17844 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DDX5 or p68, a member of DEAD/H BOX, which has a characteristic DEAD box, is a proliferation-associated nuclear antigen. DDX5 involves in the alternative regulation of pre-mRNA splicing, act as a RNA helicase to increase tau exon10 inclusion in a RBM4-dependent manner. It's also a transcription coactivator for extrogen receptor ESR1 and androgen receptor AR. Once synergized with DDX17 and SRA1 RNA, DDX5 activated MYOD1 transcription activity and involved in skeletal muscle differentiation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DDX5 antibody 67025-1-Ig | Download protocol |
| IHC protocol for DDX5 antibody 67025-1-Ig | Download protocol |
| IF protocol for DDX5 antibody 67025-1-Ig | Download protocol |
| IP protocol for DDX5 antibody 67025-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Theranostics LncRNA PRADX-mediated recruitment of PRC2/DDX5 complex suppresses UBXN1 expression and activates NF-κB activity, promoting tumorigenesis. | ||
Cancer Biol Ther VAMP8 suppresses the metastasis via DDX5/β-catenin signal pathway in osteosarcoma | ||
Virol J DEAD box RNA helicase 5 is a new pro-viral host factor for Sindbis virus infection | ||
J Am Chem Soc Polymerase η Recruits DHX9 Helicase to Promote Replication across Guanine Quadruplex Structures |
