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CoraLite® Plus 488-conjugated DDX5 Monoclonal antibody

DDX5 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D3D2

Cat no : CL488-67025

Print datasheet

Synonyms

HLR1, HELR, G17P1, EC:3.6.4.13, DDX5,p68

验证数据展示

  • IF Staining of HepG2 using CL488-67025

    IF Staining of HepG2 using CL488-67025

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488-conjugated DDX5 antibody (CL488-67025, Clone: 2D3D2 ) at dilution of 1:100.

  • FC experiment of HeLa using CL488-67025

    FC experiment of HeLa using CL488-67025

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human DDX5 (CL488-67025, Clone:2D3D2) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DDX5 Ready-To-Use IHC Kit. DDX5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67025-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67025 targets DDX5 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen DDX5 fusion protein Ag24821 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
Synonyms HLR1, HELR, G17P1, EC:3.6.4.13, DDX5,p68
Observed Molecular Weight 69 kDa
GenBank Accession NumberNM_004396
Gene Symbol DDX5
Gene ID (NCBI) 1655
RRIDAB_2919407
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP17844
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DDX5 antibody CL488-67025Download protocol
FC protocol for CL Plus 488 DDX5 antibody CL488-67025Download protocol
Standard Protocols
Click here to view our Standard Protocols