DDX5 Monoclonal antibody, PBS Only

Various lysates were subjected to SDS PAGE followed by western blot with 67025-1-Ig (DDX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67025-1-PBS in a different storage buffer formulation.

IP result of anti-DDX5 (IP:67025-1-Ig, 5ug; Detection:67025-1-Ig 1:1000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 67025-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67025-1-Ig (DDX5 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67025-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX5 antibody (67025-1-Ig, Clone: 2D3D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67025-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 67025-1-Ig (DDX5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67025-1-PBS in a different storage buffer formulation.