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Host
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Species Reactivity
0
Species Reactivity
0
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Compatible Species
0
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验证数据展示

  • WB analysis using 82969-1-RR (same clone as 82969-1-PBS)

    WB analysis using 82969-1-RR (same clone as 82969-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82969-1-RR (DDX60 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82969-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 82969-1-RR (same clone as 82969-1-PBS)

    IHC staining of human stomach using 82969-1-RR (same clone as 82969-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82969-1-RR (DDX60 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82969-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 82969-1-RR (same clone as 82969-1-PBS)

    FC experiment of A431 using 82969-1-RR (same clone as 82969-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.2 ug DDX60 Recombinant antibody (82969-1-RR, Clone:230293C10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82969-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 82969-1-RR (same clone as 82969-1-PBS)

    IF Staining of A431 using 82969-1-RR (same clone as 82969-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DDX60 antibody (82969-1-RR, Clone: 230293C10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82969-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82969-1-RR

    Affinity and Kinetic Characterization of 82969-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82969-1-RR against Human DDX60 were performed. The affinity constant is 0.145 nM.

DDX60 Recombinant antibody, PBS Only

DDX60 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82969-1-PBS

产品说明书

CloneNo. 230293C10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

Probable ATP-dependent RNA helicase DDX60, EC:3.6.4.13, DEAD box protein 60, 230293C10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82969-1-PBS targets DDX60 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 DDX60 fusion protein Ag34406 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
别名 Probable ATP-dependent RNA helicase DDX60, EC:3.6.4.13, DEAD box protein 60, 230293C10
计算分子量 1712 aa, 198 kDa
观测分子量170-200 kDa
GenBank蛋白编号BC038115
基因名称 DDX60
Gene ID (NCBI) 55601
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IY21
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX60, also called FLJ20035, is an IFN-inducible gene that has been identified via a microarray analysis of genes induced by viral infection in human dendritic cells (DCs). DDX60 expression correlated strongly with immune checkpoint and immune system-related metagene clusters, and DDX60 promoted cell proliferation, migration, and invasion and was related to poor prognosis and immune resistance(PMID: 37274827). Involved in RIG-I-dependent and independent innate immune responses, DDX60 has been proven to be associated with the development of tumors.