验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HEK-293 cells, U-87 MG cells, U-251 cells |
Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A431 cells |
Positive FC (Intra) detected in | A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82969-1-RR targets DDX60 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
经测试反应性 | human |
免疫原 | DDX60 fusion protein Ag34406 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 60 |
别名 | Probable ATP-dependent RNA helicase DDX60, EC:3.6.4.13, DEAD box protein 60, 230293C10 |
计算分子量 | 1712 aa, 198 kDa |
观测分子量 | 170-200 kDa |
GenBank蛋白编号 | BC038115 |
基因名称 | DDX60 |
Gene ID (NCBI) | 55601 |
RRID | AB_3670716 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q8IY21 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DDX60, also called FLJ20035, is an IFN-inducible gene that has been identified via a microarray analysis of genes induced by viral infection in human dendritic cells (DCs). DDX60 expression correlated strongly with immune checkpoint and immune system-related metagene clusters, and DDX60 promoted cell proliferation, migration, and invasion and was related to poor prognosis and immune resistance(PMID: 37274827). Involved in RIG-I-dependent and independent innate immune responses, DDX60 has been proven to be associated with the development of tumors.
实验方案
Product Specific Protocols | |
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WB protocol for DDX60 antibody 82969-1-RR | Download protocol |
IHC protocol for DDX60 antibody 82969-1-RR | Download protocol |
IF protocol for DDX60 antibody 82969-1-RR | Download protocol |
FC protocol for DDX60 antibody 82969-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |