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DDX60 Recombinant antibody

DDX60 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230293C10

Cat no : 82969-1-RR

Print datasheet

Synonyms

Probable ATP-dependent RNA helicase DDX60, EC:3.6.4.13, DEAD box protein 60, 230293C10

验证数据展示

  • WB analysis using 82969-1-RR

    WB analysis using 82969-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82969-1-RR (DDX60 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 82969-1-RR

    IHC staining of human stomach using 82969-1-RR

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82969-1-RR (DDX60 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of A431 using 82969-1-RR

    FC experiment of A431 using 82969-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.2 ug DDX60 Recombinant antibody (82969-1-RR, Clone:230293C10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of A431 using 82969-1-RR

    IF Staining of A431 using 82969-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DDX60 antibody (82969-1-RR, Clone: 230293C10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Affinity and Kinetic Characterization of 82969-1-RR

    Affinity and Kinetic Characterization of 82969-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82969-1-RR against Human DDX60 were performed. The affinity constant is 0.145 nM.

经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, U-87 MG cells, U-251 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82969-1-RR targets DDX60 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen DDX60 fusion protein Ag34406 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
Synonyms Probable ATP-dependent RNA helicase DDX60, EC:3.6.4.13, DEAD box protein 60, 230293C10
Calculated Molecular Weight 1712 aa, 198 kDa
Observed Molecular Weight170-200 kDa
GenBank Accession NumberBC038115
Gene Symbol DDX60
Gene ID (NCBI) 55601
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IY21
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX60, also called FLJ20035, is an IFN-inducible gene that has been identified via a microarray analysis of genes induced by viral infection in human dendritic cells (DCs). DDX60 expression correlated strongly with immune checkpoint and immune system-related metagene clusters, and DDX60 promoted cell proliferation, migration, and invasion and was related to poor prognosis and immune resistance(PMID: 37274827). Involved in RIG-I-dependent and independent innate immune responses, DDX60 has been proven to be associated with the development of tumors.

实验方案

Product Specific Protocols
WB protocol for DDX60 antibody 82969-1-RRDownload protocol
IHC protocol for DDX60 antibody 82969-1-RRDownload protocol
IF protocol for DDX60 antibody 82969-1-RRDownload protocol
FC protocol for DDX60 antibody 82969-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols