DDX60 Recombinant antibody

DDX60 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 82969-1-RR

产品说明书

CloneNo. 230293C10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

Probable ATP-dependent RNA helicase DDX60, EC:3.6.4.13, DEAD box protein 60, 230293C10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, U-87 MG cells, U-251 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82969-1-RR targets DDX60 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 DDX60 fusion protein Ag34406 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
别名 Probable ATP-dependent RNA helicase DDX60, EC:3.6.4.13, DEAD box protein 60, 230293C10
计算分子量 1712 aa, 198 kDa
观测分子量170-200 kDa
GenBank蛋白编号BC038115
基因名称 DDX60
Gene ID (NCBI) 55601
RRIDAB_3670716
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IY21
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX60, also called FLJ20035, is an IFN-inducible gene that has been identified via a microarray analysis of genes induced by viral infection in human dendritic cells (DCs). DDX60 expression correlated strongly with immune checkpoint and immune system-related metagene clusters, and DDX60 promoted cell proliferation, migration, and invasion and was related to poor prognosis and immune resistance(PMID: 37274827). Involved in RIG-I-dependent and independent innate immune responses, DDX60 has been proven to be associated with the development of tumors.

实验方案

Product Specific Protocols
WB protocol for DDX60 antibody 82969-1-RRDownload protocol
IHC protocol for DDX60 antibody 82969-1-RRDownload protocol
IF protocol for DDX60 antibody 82969-1-RRDownload protocol
FC protocol for DDX60 antibody 82969-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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