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验证数据展示

  • IHC staining of human lung cancer using 82969-4-RR (same clone as 82969-4-PBS)

    IHC staining of human lung cancer using 82969-4-RR (same clone as 82969-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82969-4-RR (DDX60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82969-4-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 82969-4-RR (same clone as 82969-4-PBS)

    IHC staining of human lung cancer using 82969-4-RR (same clone as 82969-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82969-4-RR (DDX60 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82969-4-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 82969-4-RR (same clone as 82969-4-PBS)

    FC experiment of A431 using 82969-4-RR (same clone as 82969-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human DDX60 (82969-4-RR, Clone:230293G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82969-4-PBS in a different storage buffer formulation.

DDX60 Recombinant antibody, PBS Only

DDX60 Uni-rAbTM Recombinant Antibody for IHC, FC (Intra), Indirect ELISA
Cat No. 82969-4-PBS

Print datasheet

CloneNo. 230293G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC (Intra), Indirect ELISA

Synonyms

Probable ATP-dependent RNA helicase DDX60, EC:3.6.4.13, DEAD box protein 60, 230293G10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

82969-4-PBS targets DDX60 in IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications IHC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen DDX60 fusion protein Ag34406 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
Synonyms Probable ATP-dependent RNA helicase DDX60, EC:3.6.4.13, DEAD box protein 60, 230293G10
Calculated Molecular Weight 1712 aa, 198 kDa
Observed Molecular Weight 170-200 kDa
GenBank Accession NumberBC038115
Gene Symbol DDX60
Gene ID (NCBI) 55601
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IY21
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX60, also called FLJ20035, is an IFN-inducible gene that has been identified via a microarray analysis of genes induced by viral infection in human dendritic cells (DCs). DDX60 expression correlated strongly with immune checkpoint and immune system-related metagene clusters, and DDX60 promoted cell proliferation, migration, and invasion and was related to poor prognosis and immune resistance(PMID: 37274827). Involved in RIG-I-dependent and independent innate immune responses, DDX60 has been proven to be associated with the development of tumors.