• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IHC staining of human lung cancer using 82969-4-RR (same clone as 82969-4-PBS)

    IHC staining of human lung cancer using 82969-4-RR (same clone as 82969-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82969-4-RR (DDX60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82969-4-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 82969-4-RR (same clone as 82969-4-PBS)

    IHC staining of human lung cancer using 82969-4-RR (same clone as 82969-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82969-4-RR (DDX60 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82969-4-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 82969-4-RR (same clone as 82969-4-PBS)

    FC experiment of A431 using 82969-4-RR (same clone as 82969-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human DDX60 (82969-4-RR, Clone:230293G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82969-4-PBS in a different storage buffer formulation.

DDX60 Recombinant antibody, PBS Only

DDX60 Uni-rAbTM Recombinant Antibody for IHC, FC (Intra), Indirect ELISA
Cat No. 82969-4-PBS

产品说明书

CloneNo. 230293G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, FC (Intra), Indirect ELISA

别名

Probable ATP-dependent RNA helicase DDX60, EC:3.6.4.13, DEAD box protein 60, 230293G10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

82969-4-PBS targets DDX60 in IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 DDX60 fusion protein Ag34406 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
别名 Probable ATP-dependent RNA helicase DDX60, EC:3.6.4.13, DEAD box protein 60, 230293G10
计算分子量 1712 aa, 198 kDa
观测分子量 170-200 kDa
GenBank蛋白编号BC038115
基因名称 DDX60
Gene ID (NCBI) 55601
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IY21
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX60, also called FLJ20035, is an IFN-inducible gene that has been identified via a microarray analysis of genes induced by viral infection in human dendritic cells (DCs). DDX60 expression correlated strongly with immune checkpoint and immune system-related metagene clusters, and DDX60 promoted cell proliferation, migration, and invasion and was related to poor prognosis and immune resistance(PMID: 37274827). Involved in RIG-I-dependent and independent innate immune responses, DDX60 has been proven to be associated with the development of tumors.