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DDX60 Recombinant antibody

DDX60 Uni-rAbTM Recombinant Antibody for IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230293G10

Cat no : 82969-4-RR

Print datasheet

Synonyms

Probable ATP-dependent RNA helicase DDX60, EC:3.6.4.13, DEAD box protein 60, 230293G10

验证数据展示

  • FC experiment of A431 using 82969-4-RR

    FC experiment of A431 using 82969-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human DDX60 (82969-4-RR, Clone:230293G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human lung cancer using 82969-4-RR

    IHC staining of human lung cancer using 82969-4-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82969-4-RR (DDX60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 82969-4-RR

    IHC staining of human lung cancer using 82969-4-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82969-4-RR (DDX60 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA431 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82969-4-RR targets DDX60 in IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IHC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen DDX60 fusion protein Ag34406 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
Synonyms Probable ATP-dependent RNA helicase DDX60, EC:3.6.4.13, DEAD box protein 60, 230293G10
Calculated Molecular Weight 1712 aa, 198 kDa
Observed Molecular Weight 170-200 kDa
GenBank Accession NumberBC038115
Gene Symbol DDX60
Gene ID (NCBI) 55601
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IY21
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX60, also called FLJ20035, is an IFN-inducible gene that has been identified via a microarray analysis of genes induced by viral infection in human dendritic cells (DCs). DDX60 expression correlated strongly with immune checkpoint and immune system-related metagene clusters, and DDX60 promoted cell proliferation, migration, and invasion and was related to poor prognosis and immune resistance(PMID: 37274827). Involved in RIG-I-dependent and independent innate immune responses, DDX60 has been proven to be associated with the development of tumors.

实验方案

Product Specific Protocols
IHC protocol for DDX60 antibody 82969-4-RRDownload protocol
FC protocol for DDX60 antibody 82969-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols