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DEAF1 Polyclonal antibody

DEAF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18323-1-AP

Print datasheet

Synonyms

ZMYND5, Zinc finger MYND domain-containing protein 5, Suppressin, SPN, NUDR

验证数据展示

  • WB analysis using 18323-1-AP

    WB analysis using 18323-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18323-1-AP (DEAF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 18323-1-AP

    IHC staining of human kidney using 18323-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DEAF1 antibody included
  • IHC staining of human kidney using 18323-1-AP

    IHC staining of human kidney using 18323-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DEAF1 antibody included
  • IF Staining of Jurkat using 18323-1-AP

    IF Staining of Jurkat using 18323-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DEAF1 antibody (18323-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, BxPC-3 cells, Jurkat cells, SH-SY5Y cells
Positive IHC detected inhuman kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, human spleen tissue, human lung tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy DEAF1 Ready-To-Use IHC Kit. DEAF1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18323-1-AP targets DEAF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DEAF1 fusion protein Ag13173 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name deformed epidermal autoregulatory factor 1 (Drosophila)
Synonyms ZMYND5, Zinc finger MYND domain-containing protein 5, Suppressin, SPN, NUDR
Calculated Molecular Weight 565 aa, 59 kDa
Observed Molecular Weight 60-63 kDa, 72 kDa
GenBank Accession NumberBC053322
Gene Symbol DEAF1
Gene ID (NCBI) 10522
RRIDAB_2261595
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75398
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DEAF1 antibody 18323-1-APDownload protocol
IHC protocol for DEAF1 antibody 18323-1-APDownload protocol
IF protocol for DEAF1 antibody 18323-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols