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验证数据展示

  • WB analysis using 18323-1-AP

    WB analysis using 18323-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18323-1-AP (DEAF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 18323-1-AP

    IHC staining of human kidney using 18323-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DEAF1 antibody included
  • IHC staining of human kidney using 18323-1-AP

    IHC staining of human kidney using 18323-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DEAF1 antibody included
  • IF Staining of Jurkat using 18323-1-AP

    IF Staining of Jurkat using 18323-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DEAF1 antibody (18323-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

DEAF1 Polyclonal antibody

DEAF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18323-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ZMYND5, Zinc finger MYND domain-containing protein 5, Suppressin, SPN, NUDR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, BxPC-3 cells, Jurkat cells, SH-SY5Y cells
Positive IHC detected inhuman kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, human spleen tissue, human lung tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy DEAF1 Ready-To-Use IHC Kit. DEAF1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18323-1-AP targets DEAF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DEAF1 fusion protein Ag13173 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 deformed epidermal autoregulatory factor 1 (Drosophila)
别名 ZMYND5, Zinc finger MYND domain-containing protein 5, Suppressin, SPN, NUDR
计算分子量 565 aa, 59 kDa
观测分子量 60-63 kDa, 72 kDa
GenBank蛋白编号BC053322
基因名称 DEAF1
Gene ID (NCBI) 10522
RRIDAB_2261595
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75398
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DEAF1 antibody 18323-1-APDownload protocol
IHC protocol for DEAF1 antibody 18323-1-APDownload protocol
IF protocol for DEAF1 antibody 18323-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols