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CoraLite® Plus 488-conjugated DEAF1 Polyclonal antibody

DEAF1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-18323

Print datasheet

Synonyms

验证数据展示

  • IF Staining of Jurkat using CL488-18323

    IF Staining of Jurkat using CL488-18323

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 DEAF1 antibody (CL488-18323) at dilution of 1:200.

  • FC experiment of Jurkat using CL488-18323

    FC experiment of Jurkat using CL488-18323

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Deaf1 Polyclonal Antibody (CL488-18323)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy DEAF1 Ready-To-Use IHC Kit. DEAF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 18323-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-18323 targets DEAF1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen DEAF1 fusion protein Ag13173 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name deformed epidermal autoregulatory factor 1 (Drosophila)
Synonyms
Calculated Molecular Weight 565 aa, 59 kDa
Observed Molecular Weight 60-63 kDa, 72 kDa
GenBank Accession NumberBC053322
Gene Symbol DEAF1
Gene ID (NCBI) 10522
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75398
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DEAF1 antibody CL488-18323Download protocol
FC protocol for CL Plus 488 DEAF1 antibody CL488-18323Download protocol
Standard Protocols
Click here to view our Standard Protocols