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  • Featured Product
  • KD/KO Validated

DEK Monoclonal antibody, PBS Only

DEK Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5E3A6

Cat no : 66194-1-PBS

Print datasheet

Synonyms

D6S231E, 5E3A6

验证数据展示

  • WB analysis using 66194-1-Ig (same clone as 66194-1-PBS)

    WB analysis using 66194-1-Ig (same clone as 66194-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66194-1-Ig (DEK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66194-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66194-1-Ig (same clone as 66194-1-PBS)

    WB analysis of HEK-293 using 66194-1-Ig (same clone as 66194-1-PBS)

    WB result of DEK antibody (66194-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DEK transfected HEK-293 cells. This data was developed using the same antibody clone with 66194-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 66194-1-Ig (same clone as 66194-1-PBS)

    IHC staining of human placenta using 66194-1-Ig (same clone as 66194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66194-1-Ig (DEK antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66194-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DEK antibody included
  • IHC staining of human placenta using 66194-1-Ig (same clone as 66194-1-PBS)

    IHC staining of human placenta using 66194-1-Ig (same clone as 66194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66194-1-Ig (DEK antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66194-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DEK antibody included
  • IF Staining of HepG2 using 66194-1-Ig (same clone as 66194-1-PBS)

    IF Staining of HepG2 using 66194-1-Ig (same clone as 66194-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DEK antibody (66194-1-Ig, Clone: 5E3A6 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66194-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy DEK Ready-To-Use IHC Kit. DEK primary antibody included.

产品信息

66194-1-PBS targets DEK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DEK fusion protein Ag8225 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name DEK oncogene
Synonyms D6S231E, 5E3A6
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberBC035259
Gene Symbol DEK
Gene ID (NCBI) 7913
RRIDAB_2881588
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35659
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transdcution. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.