• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66194-1-Ig (same clone as 66194-1-PBS)

    WB analysis using 66194-1-Ig (same clone as 66194-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66194-1-Ig (DEK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66194-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66194-1-Ig (same clone as 66194-1-PBS)

    WB analysis of HEK-293 using 66194-1-Ig (same clone as 66194-1-PBS)

    WB result of DEK antibody (66194-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DEK transfected HEK-293 cells. This data was developed using the same antibody clone with 66194-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 66194-1-Ig (same clone as 66194-1-PBS)

    IHC staining of human placenta using 66194-1-Ig (same clone as 66194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66194-1-Ig (DEK antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66194-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DEK antibody included
  • IHC staining of human placenta using 66194-1-Ig (same clone as 66194-1-PBS)

    IHC staining of human placenta using 66194-1-Ig (same clone as 66194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66194-1-Ig (DEK antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66194-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DEK antibody included
  • IF Staining of HepG2 using 66194-1-Ig (same clone as 66194-1-PBS)

    IF Staining of HepG2 using 66194-1-Ig (same clone as 66194-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DEK antibody (66194-1-Ig, Clone: 5E3A6 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66194-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

DEK Monoclonal antibody, PBS Only

DEK Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66194-1-PBS

产品说明书

CloneNo. 5E3A6

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

D6S231E, 5E3A6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy DEK Ready-To-Use IHC Kit. DEK primary antibody included.

产品信息

66194-1-PBS targets DEK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DEK fusion protein Ag8225 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 DEK oncogene
别名 D6S231E, 5E3A6
计算分子量 43 kDa
观测分子量 40-50 kDa
GenBank蛋白编号BC035259
基因名称 DEK
Gene ID (NCBI) 7913
RRIDAB_2881588
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35659
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transdcution. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.