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- KD/KO Validated
DEK Monoclonal antibody
DEK Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
5E3A6
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, Raji cells, Ramos cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy DEK Ready-To-Use IHC Kit. DEK primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66194-1-Ig targets DEK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | DEK fusion protein Ag8225 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | DEK oncogene |
| Synonyms | D6S231E, 5E3A6 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 40-50 kDa |
| GenBank Accession Number | BC035259 |
| Gene Symbol | DEK |
| Gene ID (NCBI) | 7913 |
| RRID | AB_2881588 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P35659 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transdcution. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DEK antibody 66194-1-Ig | Download protocol |
| IHC protocol for DEK antibody 66194-1-Ig | Download protocol |
| IF protocol for DEK antibody 66194-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Exp Med Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis. | ||
Biochem J DEK terminates diapause by activation of quiescent cells in the crustacean Artemia.
| ||
Mol Med Rep Overexpression of DEK gene is correlated with poor prognosis in hepatocellular carcinoma. |