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验证数据展示

  • WB analysis using CL594-66194

    WB analysis using CL594-66194

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-66194 (DEK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using CL594-66194

    FC experiment of HeLa using CL594-66194

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human DEK (CL594-66194, Clone:5E3A6) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated DEK Monoclonal antibody

DEK Monoclonal Antibody for WB, FC (Intra)
Cat No. CL594-66194

产品说明书

CloneNo. 5E3A6

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, FC (Intra)

别名

D6S231E, 5E3A6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DEK Ready-To-Use IHC Kit. DEK primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66194-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66194 targets DEK in WB, FC (Intra) applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human
免疫原 DEK fusion protein Ag8225 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 DEK oncogene
别名 D6S231E, 5E3A6
计算分子量 43 kDa
观测分子量 50 kDa
GenBank蛋白编号BC035259
基因名称 DEK
Gene ID (NCBI) 7913
RRIDAB_2919963
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35659
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transdcution. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.

实验方案

Product Specific Protocols
WB protocol for CL594 DEK antibody CL594-66194Download protocol
Standard Protocols
Click here to view our Standard Protocols