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验证数据展示

  • WB analysis using 82880-1-RR

    WB analysis using 82880-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82880-1-RR (DEK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 82880-1-RR

    WB analysis of HEK-293 using 82880-1-RR

    WB result of DEK antibody (82880-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DEK transfected HEK-293 cells.

  • IF Staining of U2OS using 82880-1-RR

    IF Staining of U2OS using 82880-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DEK antibody (82880-1-RR, Clone: 230065H2 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • IF Staining of HeLa using 82880-1-RR

    IF Staining of HeLa using 82880-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DEK antibody (82880-1-RR, Clone: 230065H2 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • FC experiment of HeLa using 82880-1-RR

    FC experiment of HeLa using 82880-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DEK Recombinant antibody (82880-1-RR, Clone:230065H2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IHC staining of human skin cancer using 82880-1-RR

    IHC staining of human skin cancer using 82880-1-RR

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82880-1-RR (DEK antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DEK antibody included
  • Affinity and Kinetic Characterization of 82880-1-RR

    Affinity and Kinetic Characterization of 82880-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82880-1-RR against Human DEK were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

DEK Recombinant antibody

DEK Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 82880-1-RR

产品说明书

CloneNo. 230065H2

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

D6S231E, 230065H2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, HeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DEK Ready-To-Use IHC Kit. DEK primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82880-1-RR targets DEK in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原 DEK fusion protein Ag8225 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEK oncogene
别名 D6S231E, 230065H2
计算分子量 43 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC035259
基因名称 DEK
Gene ID (NCBI) 7913
RRIDAB_3670599
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35659
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transdcution. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.

实验方案

Product Specific Protocols
WB protocol for DEK antibody 82880-1-RRDownload protocol
IHC protocol for DEK antibody 82880-1-RRDownload protocol
IF protocol for DEK antibody 82880-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols