验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy DEK Ready-To-Use IHC Kit. DEK primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82880-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82880 targets DEK in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human, rat |
| 免疫原 | DEK fusion protein Ag8225 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | DEK oncogene |
| 别名 | D6S231E, 230065H2 |
| 计算分子量 | 43 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC035259 |
| 基因名称 | DEK |
| Gene ID (NCBI) | 7913 |
| RRID | AB_3673095 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P35659 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transdcution. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 DEK antibody CL488-82880 | Download protocol |
| FC protocol for CL Plus 488 DEK antibody CL488-82880 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |