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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DEK Recombinant antibody

DEK Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230065H2

Cat no : CL488-82880

Print datasheet

Synonyms

D6S231E, 230065H2

验证数据展示

  • IF Staining of HepG2 using CL488-82880

    IF Staining of HepG2 using CL488-82880

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 DEK antibody (CL488-82880, Clone: 230065H2 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-82880

    FC experiment of HeLa using CL488-82880

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated DEK Recombinant antibody (CL488-82880, Clone:230065H2)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DEK Ready-To-Use IHC Kit. DEK primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82880-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82880 targets DEK in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, rat
Immunogen DEK fusion protein Ag8225 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name DEK oncogene
Synonyms D6S231E, 230065H2
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC035259
Gene Symbol DEK
Gene ID (NCBI) 7913
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35659
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transdcution. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DEK antibody CL488-82880Download protocol
FC protocol for CL Plus 488 DEK antibody CL488-82880Download protocol
Standard Protocols
Click here to view our Standard Protocols