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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67731-1-Ig (same clone as 67731-1-PBS)

    WB analysis using 67731-1-Ig (same clone as 67731-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67731-1-Ig (DFNA5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67731-1-PBS in a different storage buffer formulation.

  • WB analysis using 67731-1-Ig (same clone as 67731-1-PBS)

    WB analysis using 67731-1-Ig (same clone as 67731-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67731-1-Ig (DFNA5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67731-1-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 67731-1-Ig (same clone as 67731-1-PBS)

    FC experiment of SH-SY5Y using 67731-1-Ig (same clone as 67731-1-PBS)

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human DFNA5/GSDME (67731-1-Ig, Clone:1D9C3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67731-1-PBS in a different storage buffer formulation.

DFNA5/GSDME Monoclonal antibody, PBS Only

DFNA5/GSDME Monoclonal Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 67731-1-PBS

产品说明书

CloneNo. 1D9C3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, FC (Intra), Indirect ELISA

别名

DFNA5, Gasdermin E, Gasdermin-E, GSDME, 1D9C3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy GSDME/DFNA5 Ready-To-Use IHC Kit. GSDME/DFNA5 primary antibody included.

产品信息

67731-1-PBS targets DFNA5/GSDME in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 DFNA5/GSDME fusion protein Ag30514 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 deafness, autosomal dominant 5
别名 DFNA5, Gasdermin E, Gasdermin-E, GSDME, 1D9C3
计算分子量 496 aa, 55 kDa
观测分子量50-55 kDa
GenBank蛋白编号BC019689
基因名称 DFNA5
Gene ID (NCBI) 1687
RRIDAB_2882917
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO60443
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain.