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DFNA5 Monoclonal antibody, PBS Only

DFNA5 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D9C3

Cat no : 67731-1-PBS

Print datasheet

Synonyms

Gasdermin-E, Gasdermin E, DFNA5/GSDME, GSDME, DFNA 5

验证数据展示

  • WB analysis using 67731-1-Ig (same clone as 67731-1-PBS)

    WB analysis using 67731-1-Ig (same clone as 67731-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67731-1-Ig (DFNA5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67731-1-PBS in a different storage buffer formulation.

  • WB analysis using 67731-1-Ig (same clone as 67731-1-PBS)

    WB analysis using 67731-1-Ig (same clone as 67731-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67731-1-Ig (DFNA5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67731-1-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 67731-1-Ig (same clone as 67731-1-PBS)

    FC experiment of SH-SY5Y using 67731-1-Ig (same clone as 67731-1-PBS)

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human DFNA5/GSDME (67731-1-Ig, Clone:1D9C3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67731-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy GSDME/DFNA5 Ready-To-Use IHC Kit. GSDME/DFNA5 primary antibody included.

产品信息

67731-1-PBS targets DFNA5 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen DFNA5 fusion protein Ag30514 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name deafness, autosomal dominant 5
Synonyms Gasdermin-E, Gasdermin E, DFNA5/GSDME, GSDME, DFNA 5
Calculated Molecular Weight 496 aa, 55 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC019689
Gene Symbol DFNA5
Gene ID (NCBI) 1687
RRIDAB_2882917
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60443
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain.