DFNA5/GSDME Monoclonal antibody

DFNA5/GSDME Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 67731-1-Ig

产品说明书

CloneNo. 1D9C3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, FC (Intra), ELISA and More (1)

DFNA5, Gasdermin E, Gasdermin-E, GSDME, 1D9C3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inpig brain tissue, HeLa cells, HEK-293 cells, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy GSDME/DFNA5 Ready-To-Use IHC Kit. GSDME/DFNA5 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67731-1-Ig targets DFNA5/GSDME in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, FC (Intra), ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat, pig
文献引用反应性human, mouse, rat
免疫原 DFNA5/GSDME fusion protein Ag30514 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 deafness, autosomal dominant 5
别名 DFNA5, Gasdermin E, Gasdermin-E, GSDME, 1D9C3
计算分子量 496 aa, 55 kDa
观测分子量50-55 kDa
GenBank蛋白编号BC019689
基因名称 DFNA5
Gene ID (NCBI) 1687
RRIDAB_2882917
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO60443
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain.

实验方案

Product Specific Protocols
WB protocol for DFNA5/GSDME antibody 67731-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

ACS Appl Mater Interfaces

Mitochondria-Targeting Pyroptosis Amplifier of Lonidamine-Modified Black Phosphorus Nanosheets for Glioblastoma Treatments

Authors - Youqing Ye
human,ratWB

Exp Neurol

SNAP25 ameliorates postoperative cognitive dysfunction by facilitating PINK1-dependent mitophagy and impeding caspase-3/GSDME-dependent pyroptosis

Authors - Wei Wang
humanWB,IHC

Cancer Cell Int

Comprehensive analysis, diagnosis, prognosis, and cordycepin (CD) regulations for GSDME expressions in pan-cancers

Authors - Jiewen Fu
human,ratWB

ACS Chem Neurosci

Inhibition of PINK1-Mediated Mitophagy Contributes to Postoperative Cognitive Dysfunction through Activation of Caspase-3/GSDME-Dependent Pyroptosis

Authors - Wei Wang
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