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CoraLite® Plus 488-conjugated DFNA5 Monoclonal antibody

DFNA5 Monoclonal Antibody for WB, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D9C3

Cat no : CL488-67731

Print datasheet

Synonyms

Gasdermin-E, Gasdermin E, DFNA5/GSDME, GSDME, DFNA 5

验证数据展示

  • WB analysis using CL488-67731

    WB analysis using CL488-67731

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-67731 (DFNA5/GSDME antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • FC experiment of SH-SY5Y using CL488-67731

    FC experiment of SH-SY5Y using CL488-67731

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DFNA5/GSDME (CL488-67731, Clone:1D9C3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy GSDME/DFNA5 Ready-To-Use IHC Kit. GSDME/DFNA5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67731-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67731 targets DFNA5 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen DFNA5 fusion protein Ag30514 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name deafness, autosomal dominant 5
Synonyms Gasdermin-E, Gasdermin E, DFNA5/GSDME, GSDME, DFNA 5
Calculated Molecular Weight 496 aa, 55 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC019689
Gene Symbol DFNA5
Gene ID (NCBI) 1687
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO60443
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 DFNA5 antibody CL488-67731Download protocol
Standard Protocols
Click here to view our Standard Protocols