CoraLite® Plus 488-conjugated DFNA5/GSDME Monoclonal antibody
DFNA5/GSDME Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat, pig
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1D9C3
验证数据展示
经过测试的应用
Positive FC detected in | SH-SY5Y cells |
Planning an IHC experiment? We recommend our IHCeasy GSDME/DFNA5 Ready-To-Use IHC Kit. GSDME/DFNA5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67731-1-Ig
推荐稀释比
Application | Dilution |
---|---|
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67731 targets DFNA5/GSDME in FC (Intra) applications and shows reactivity with Human, mouse, rat, pig samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | Human, mouse, rat, pig |
Immunogen | DFNA5/GSDME fusion protein Ag30514 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | deafness, autosomal dominant 5 |
Synonyms | deafness, autosomal dominant 5, DFNA5, GSDME, ICERE 1, ICERE1 |
Calculated Molecular Weight | 496 aa, 55 kDa |
Observed Molecular Weight | 50-55 kDa |
GenBank Accession Number | BC019689 |
Gene Symbol | DFNA5 |
Gene ID (NCBI) | 1687 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | O60443 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain.
实验方案
Product Specific Protocols | |
---|---|
FC protocol for CL Plus 488 DFNA5/GSDME antibody CL488-67731 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |