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验证数据展示

  • IF Staining of HepG2 using 83262-2-RR

    IF Staining of HepG2 using 83262-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DGAT2 antibody (83262-2-RR, Clone: 240171C6 ) at dilution of 1:150 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 83262-2-RR

    FC experiment of HeLa using 83262-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Dgat2 Recombinant Antibody (83262-2-RR, Clone:240171C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

DGAT2 Recombinant antibody

DGAT2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83262-2-RR

Print datasheet

CloneNo. 240171C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

EC:2.3.1.20, Diglyceride acyltransferase 2, Diacylglycerol O-acyltransferase 2, ARAT, Acyl-CoA retinol O-fatty-acyltransferase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83262-2-RR targets DGAT2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen DGAT2 fusion protein Ag10719 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name diacylglycerol O-acyltransferase homolog 2 (mouse)
Synonyms EC:2.3.1.20, Diglyceride acyltransferase 2, Diacylglycerol O-acyltransferase 2, ARAT, Acyl-CoA retinol O-fatty-acyltransferase
Calculated Molecular Weight 388 aa, 44 kDa
GenBank Accession NumberBC015234
Gene Symbol DGAT2
Gene ID (NCBI) 84649
RRIDAB_3670933
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96PD7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Diacylglycerol acyltransferase 2 (DGAT2) is an integral membrane protein and major enzyme that catalyzes the final step of triacylglycerol (TG) synthesis in eukaryotes (PMID: 21680734). DGAT2 is present in mitochondria-associated membranes, specialized domains of the ER that are highly enriched in lipid synthetic enzymes (PMID: 19049983). DGAT2 isoforms (39 kDa, 44kDa) have major differences in acyl-CoA substrate specificity toward erucoyl-CoA.

实验方案

Product Specific Protocols
IF protocol for DGAT2 antibody 83262-2-RRDownload protocol
FC protocol for DGAT2 antibody 83262-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols