验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-83262-2 targets DGAT2 in IF/ICC applications and shows reactivity with human samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | human |
免疫原 | DGAT2 fusion protein Ag10719 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | diacylglycerol O-acyltransferase homolog 2 (mouse) |
别名 | EC:2.3.1.20, Diglyceride acyltransferase 2, Diacylglycerol O-acyltransferase 2, ARAT, Acyl-CoA retinol O-fatty-acyltransferase |
计算分子量 | 388 aa, 44 kDa |
GenBank蛋白编号 | BC015234 |
基因名称 | DGAT2 |
Gene ID (NCBI) | 84649 |
RRID | AB_3673229 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q96PD7 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Diacylglycerol acyltransferase 2 (DGAT2) is an integral membrane protein and major enzyme that catalyzes the final step of triacylglycerol (TG) synthesis in eukaryotes (PMID: 21680734). DGAT2 is present in mitochondria-associated membranes, specialized domains of the ER that are highly enriched in lipid synthetic enzymes (PMID: 19049983). DGAT2 isoforms (39 kDa, 44kDa) have major differences in acyl-CoA substrate specificity toward erucoyl-CoA.
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 DGAT2 antibody CL488-83262-2 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |