验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, MOLT-4 cells, human cerebellum tissue, HEK-293 cells, mouse cerebellum tissue |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human gliomas tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11547-1-AP targets DGKA in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | DGKA fusion protein Ag2075 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | diacylglycerol kinase, alpha 80kDa |
| 别名 | EC:2.7.1.107, DGK-alpha, DGK alpha, DAGK1, DAGK |
| 计算分子量 | 735 aa, 83 kDa |
| 观测分子量 | 83 kDa |
| GenBank蛋白编号 | BC023523 |
| 基因名称 | DGKA |
| Gene ID (NCBI) | 1606 |
| RRID | AB_2245857 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P23743 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DGKA(Diacylglycerol kinase alpha) is also named as DAGK, DAGK1 and belongs to the eukaryotic diacylglycerol kinase family,which is suggested to attenuate diacylglycerol-induced cell responses through the phosphorylation of this second messenger to phosphatidic acid.DGKA regulates the secretion of lethal exosomes bearing Fas ligand during activation-induced cell death of T lymphocytes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DGKA antibody 11547-1-AP | Download protocol |
| IHC protocol for DGKA antibody 11547-1-AP | Download protocol |
| IF protocol for DGKA antibody 11547-1-AP | Download protocol |
| IP protocol for DGKA antibody 11547-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Mutant p53s generate pro-invasive niches by influencing exosome podocalyxin levels. | ||
Nat Chem Biol Reprogramming fatty acyl specificity of lipid kinases via C1 domain engineering.
| ||
Nat Commun Epigenetic regulation of diacylglycerol kinase alpha promotes radiation-induced fibrosis. | ||
EMBO J Dysfunctional natural killer cells can be reprogrammed to regain anti-tumor activity | ||
Cancer Discov Diacylglycerol Kinase α Is a Critical Signaling Node and Novel Therapeutic Target in Glioblastoma and Other Cancers.
| ||
Clin Cancer Res DGKA Provides Platinum Resistance in Ovarian Cancer Through Activation of c-JUN-WEE1 Signaling.
|
